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Bethyl Laboratories
TRIM25 Polyclonal Antibody
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FIGURE: 1 / 2
TRIM25 Antibody (A301-857A) in WB
Detection of human TRIM25 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, MCF-7, LNCaP, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIM25 antibody (Product # A301-857A) (lot A301-857A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A301-857A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 325 and 375 of Human tripartite motif-containing 25.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; tripartite motif protein TRIM25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; zinc finger protein 147 (estrogen-responsive finger protein); zinc finger protein-147
Gene Aliases: EFP; RNF147; TRIM25; Z147; ZNF147
UniProt ID: (Human) Q14258
Entrez Gene ID: (Human) 7706
immune system process
cytoplasmic pattern recognition receptor signaling pathway
ubiquitin-dependent protein catabolic process
protein monoubiquitination
response to oxidative stress
mRNA transcription
protein ubiquitination
viral release from host cell
regulation of protein localization
response to vitamin D
cellular response to oxidative stress
ERAD pathway
RIG-I signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
response to estrogen
negative regulation by host of viral release from host cell
innate immune response
positive regulation of transcription, DNA-templated
regulation of viral entry into host cell
negative regulation of viral entry into host cell
defense response to virus
protein K48-linked ubiquitination
antiviral innate immune response
cellular response to leukemia inhibitory factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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