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Bethyl Laboratories
LIMK1 Polyclonal Antibody
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FIGURE: 1 / 1
LIMK1 Antibody (A302-671A) in WB
Detection of human LIMK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-LIMK1 antibody (Product # A302-671A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. LIMK1 was also immunoprecipitated by rabbit anti-LIMK1 antibody A302-670A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 5 seconds (B).
Product Details
A302-671A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 597 and 647 of human LIM domain kinase 1.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
LIMK1 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK1 has also been implicated in brain development. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.11.1; kinase LIMK1; LIM domain kinase 1; lim kinase 1; LIM motif-containing protein kinase; LIMK-1; unnamed protein product
Gene Aliases: LIMK; LIMK-1; LIMK1
UniProt ID: (Human) P53667
Entrez Gene ID: (Human) 3984
protein phosphorylation
signal transduction
Rho protein signal transduction
nervous system development
actin cytoskeleton organization
positive regulation of actin filament bundle assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
stress fiber assembly
positive regulation of axon extension
axon extension
positive regulation of stress fiber assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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