Search
Bethyl Laboratories
Vinculin Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
Vinculin Antibody (A302-534A-T) in WB
Detection of human and mouse Vinculin by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Vinculin antibody (Product # A302-534A) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A302534AT
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 750 and 800 of Human vinculin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken and Pig
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: epididymis luminal protein 114; epididymis secretory sperm binding protein; meta-vinculin; Metavinculin; MV; RP11-178G16.3; unnamed protein product; vinc; Vinculin
Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL; VINC
UniProt ID: (Human) P18206, (Mouse) Q64727
Entrez Gene ID: (Human) 7414, (Mouse) 22330
morphogenesis of an epithelium
cell adhesion
cell-matrix adhesion
lamellipodium assembly
regulation of cell migration
negative regulation of cell migration
adherens junction assembly
protein localization to cell surface
maintenance of blood-brain barrier
apical junction assembly
axon extension
regulation of focal adhesion assembly
platelet aggregation
epithelial cell-cell adhesion
regulation of establishment of endothelial barrier
regulation of protein localization to cell-cell adherens junction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support