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Bethyl Laboratories
DDX42 Polyclonal Antibody
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FIGURE: 1 / 1
DDX42 Antibody (A303-354A-T) in WB
Detection of human and mouse DDX42 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX42 antibody (Product # A303-354A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. DDX42 was also immunoprecipitated by rabbit anti-DDX42 antibody A303-353A, which recognizes an upstream epitope. Detection: Chemilu... View More
Product Details
A303-354A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 888 and 938 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 42.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Chicken and Orangutan
Target Information
This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX42; DEAD (Asp-Glu-Ala-Asp) box helicase 42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box protein 42; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD box protein; SF3b125; splicing factor 3b, subunit 8; Splicing factor 3B-associated 125 kDa protein; unnamed protein product
Gene Aliases: 1810047H21Rik; AW319508; AW556242; B430002H05Rik; DDX42; DDX42P; RHELP; RNAHP; SF3b125; SF3B8
UniProt ID: (Human) Q86XP3, (Mouse) Q810A7
Entrez Gene ID: (Human) 11325, (Mouse) 72047
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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