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Bethyl Laboratories
SKA3 Polyclonal Antibody
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FIGURE: 1 / 1
SKA3 Antibody (A304-214A-T) in IP
Detection of human SKA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody (Product # A304-214A) (lot 1) used for IP at 6 µg/mg lysate. SKA3 was also immunoprecipitated by rabbit anti-SKA3 antibody A304-215A. For blotting immunoprecipitated SKA3, A304-215A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A304-214A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 175 to 225 of Human Spindle and Kinetochore Associated Complex Subunit 3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RP11-101P17.12-002; Spindle and kinetochore-associated protein 3; unnamed protein product
Gene Aliases: C13orf3; RAMA1; SKA3
UniProt ID: (Human) Q8IX90
Entrez Gene ID: (Human) 221150
mitotic sister chromatid segregation
mitotic cell cycle
spindle assembly involved in female meiosis
chromosome segregation
mitotic metaphase plate congression
regulation of microtubule polymerization or depolymerization
establishment of meiotic spindle orientation
cell division
metaphase plate congression
attachment of mitotic spindle microtubules to kinetochore
negative regulation of mitotic spindle assembly checkpoint signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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