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Bethyl Laboratories
PAPD5/TRF4-2 Polyclonal Antibody
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FIGURE: 1 / 1
PAPD5/TRF4-2 Antibody (A305-160A-T) in IP
Detection of human PAPD5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAPD5 antibody (Product # A305-160A) (lot 1) used for IP at 6 µg per reaction. PAPD5 was also immunoprecipitated by rabbit anti-PAPD5 antibody A305-159A. For blotting immunoprecipitated PAPD5, A305-159A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A305-160A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 500 to 550 of human PAP-associated domain-containing protein 5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (PubMed:21788334, PubMed:30026317). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165). Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417). Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Non-canonical poly(A) RNA polymerase PAPD5; PAP associated domain containing 5; PAP-associated domain-containing protein 5; poly(A) RNA polymerase D5, non-canonical; Terminal guanylyltransferase; terminal nucleotidyltransferase 4A; Terminal nucleotidyltransferase 4B; Terminal uridylyltransferase 3; Topoisomerase-related function protein 4-2; TRF4-2; Trf4-2p; TUTase 3; unnamed protein product
Gene Aliases: GLD4; PAPD5; TENT4B; TRF4-2; TUT3
UniProt ID: (Human) Q8NDF8
Entrez Gene ID: (Human) 64282
rRNA processing
mRNA processing
miRNA catabolic process
RNA 3'-end processing
mRNA 3'-end processing
negative regulation of telomere maintenance via telomerase
carbohydrate homeostasis
polyadenylation-dependent ncRNA catabolic process
cell division
negative regulation of nuclear-transcribed mRNA poly(A) tail shortening
histone mRNA catabolic process
polyadenylation-dependent snoRNA 3'-end processing
positive regulation of 3'-UTR-mediated mRNA stabilization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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