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AANAT Polyclonal Antibody
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FIGURE: 1 / 4
AANAT Antibody (BS-3914R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AANAT Polyclonal Antibody, Unconjugated (bs-3914R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-3914R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Sheep,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human AANAT, amino acids 141-207.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function. Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina. Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AA-NAT; Aralkylamine N-acetyltransferase; arylakylamine N-acetyltransferase; Arylalkylamine N - acetyltransferase (Serotonin N - acetyltransferase); arylalkylamine N-acetyltransferase; pineal serotonin N-acetyltransferase; Seretonin N-acetyltransferase; Serotonin acetylase; Serotonin N-acetyltransferase
Gene Aliases: AA-NAT; AANAT; DSPS; Nat-2; Nat4; SNAT
UniProt ID: (Sheep) Q29495, (Human) Q16613, (Mouse) O88816, (Rat) Q64666
Entrez Gene ID: (Sheep) 443531, (Human) 15, (Mouse) 11298, (Rat) 25120
aralkylamine N-acetyltransferase activity
arylamine N-acetyltransferase activity
protein binding
N-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
14-3-3 protein binding
acetyltransferase
N-terminal protein amino acid acetylation
circadian rhythm
response to light stimulus
photoperiodism
response to zinc ion
melatonin biosynthetic process
response to insulin
response to cytokine
response to prostaglandin E
indolalkylamine biosynthetic process
response to copper ion
rhythmic process
response to corticosterone
response to cAMP
response to calcium ion
cellular response to cAMP
response to peptide
response to organic cyclic compound
entrainment of circadian clock by photoperiod
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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