Immunofluorescent analysis of ABCA1 (green) HEK293T cells. Cells fixed with 4% formaldehyde fixed cells were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ABCA1 polyclonal antibody (Product # PA1-16789) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Dog, Chicken, Hamster, Human, Mustelid, Mouse, Pig, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Partial peptide sequence corresponding to a sequence between residues 1100-1300 of human ABCA1.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Flow Cytometry (Flow)||1:400|
|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
ABCA1 is known to aggregate.
DO NOT BOIL SAMPLES.
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Calpains Released by T Lymphocytes Cleave TLR2 To Control IL-17 Expression.
PA1-16789 was used in western blot to elucidate the role of calpains from T lymphocytes and IL-17 expression
|Perez J,Dansou B,Hervé R,Levi C,Tamouza H,Vandermeersch S,Demey-Thomas E,Haymann JP,Zafrani L,Klatzmann D,Boissier MC,Letavernier E,Baud L||Journal of immunology (Baltimore, Md. : 1950) (196:168)||2016|
Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development.
PA1-16789 was used in western blot to study the role of xanthine oxidoreductase in macrophage foam cell formation and the effect of a xanthine oxidireductase inhibitor on atherosclerosis
|Kushiyama A,Okubo H,Sakoda H,Kikuchi T,Fujishiro M,Sato H,Kushiyama S,Iwashita M,Nishimura F,Fukushima T,Nakatsu Y,Kamata H,Kawazu S,Higashi Y,Kurihara H,Asano T||Arteriosclerosis, thrombosis, and vascular biology (32:291)||2012|
Binding of PDZ-RhoGEF to ATP-binding cassette transporter A1 (ABCA1) induces cholesterol efflux through RhoA activation and prevention of transporter degradation.
PA1-16789 was used in western blot to investigate the mechanism of cholesterol efflux mediated by ABCA1-PDZ-RhoGEF interaction
|Okuhira K,Fitzgerald ML,Tamehiro N,Ohoka N,Suzuki K,Sawada J,Naito M,Nishimaki-Mogami T||The Journal of biological chemistry (285:16369)||2010|