Immunofluorescent analysis of ABCA1 (green) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a DyLight 488-conjugated ABCA1 polyclonal antibody (Product # PA5-22908) at a dilution of 1:200 for 1 hour at room temperature in 1X PBS containing 1% BSA and 0.3% Triton X-100, and then washed with 1X PBS. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Dog, Chicken, Hamster, Human, Mustelid, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:1000|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.