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Invitrogen
ABCB4 Polyclonal Antibody
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FIGURE: 1 / 4
ABCB4 Antibody (PA5-78692) in IHC (P)
Immunohistochemistry (Paraffin) analysis of ABCB4 in paraffin-embedded section of human liver cancer tissue using ABCB4 Polyclonal Antibody (Product # PA5-78692). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight ... View More
Product Details
PA5-78692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ABCB4 recombinant protein (Position: A601-A720).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human Apolipoprotein A I shares 64% and 61.7% amino acid (aa) sequence identity with mouse and rat Apolipoprotein A I, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue. IHC: human liver cancer tissue. Flow: HepG2 cell.
Target Information
The membrane-associated protein encoded ABCB4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABC20; ATP-binding cassette sub-family B (MDR/TAP) member 4 (P-glycoprotein 3/ multidrug resistance 2; ATP-binding cassette sub-family B (MDR/TAP) member 4 (P-glycoprotein 3/ multidrug resistance 2); ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4 (P-glycoprotein 3/ multidrug resistance 2); ATP-binding cassette, subfamily B, member 4; CD243; CLCS; GP170; MDR1; MGC163296; Multidrug resistance protein 2; Multidrug resistance protein 3; multiple drug resistance 3; P glycoprotein 2; P-glycoprotein; P-glycoprotein 2; P-glycoprotein 3; P-glycoprotein 3/ multidrug resistance 2; P-glycoprotein-3/multiple drug resistance-3; P-GP; PGY1; Phosphatidylcholine translocator ABCB4
Gene Aliases: ABC21; ABCB4; GBD1; ICP3; mdr-2; MDR2; MDR2/3; MDR3; PFIC-3; Pgp3; Pgy-2; Pgy2; PGY3
UniProt ID: (Human) P21439, (Mouse) P21440, (Rat) Q08201
Entrez Gene ID: (Human) 5244, (Mouse) 18670, (Rat) 24891
nucleotide binding
protein binding
ATP binding
phospholipid transporter activity
ATPase activity
ATPase activity, coupled to transmembrane movement of substances
phosphatidylcholine-translocating ATPase activity
ceramide floppase activity
ATPase-coupled intramembrane lipid transporter activity
ABC-type transporter activity
xenobiotic-transporting ATPase activity
hydrolase activity
ATP-binding cassette (ABC) transporter
lipid metabolic process
lipid transport
positive regulation of cholesterol transport
bile acid secretion
phospholipid translocation
transmembrane transport
lipid homeostasis
positive regulation of phospholipid translocation
ceramide translocation
response to fenofibrate
cellular response to bile acid
positive regulation of phospholipid transport
transport
drug transmembrane transport
metabolic process
response to drug
xenobiotic transport
response to glucocorticoid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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