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ABH1/ALKB Polyclonal Antibody
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FIGURE: 1 / 1
ABH1/ALKB Antibody (BS-12565R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ABH1/ALKB Polyclonal Antibody, Unconjugated (bs-12565R) at 1:200 overnight at 4°C, DAB staining.
Product Details
BS-12565R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human ABH1/ALKB, amino acids 1-100.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alkB, alkylation repair homolog 1; Alkylated DNA repair protein alkB homolog 1; alkylation repair, alkB homolog; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA 6mA demethylase; DNA demethylase ALKBH1; DNA lyase ABH1; DNA N6-methyl adenine demethylase ALKBH1; DNA oxidative demethylase ALKBH1; mRNA N(3)-methylcytidine demethylase; Nucleic acid dioxygenase ALKBH1; tRNA N1-methyl adenine demethylase
Gene Aliases: 2700073G19Rik; ABH; ABH1; alkB; ALKBH; ALKBH1; hABH
UniProt ID: (Human) Q13686, (Mouse) P0CB42
Entrez Gene ID: (Human) 8846, (Rat) 362766, (Mouse) 211064
tRNA binding
catalytic activity
ferrous iron binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
lyase activity
oxidative RNA demethylase activity
oxidative DNA demethylase activity
chemoattractant activity
metal ion binding
dioxygenase activity
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
DNA N6-methyladenine demethylase activity
tRNA demethylase activity
DNA-(apurinic or apyrimidinic site) lyase activity
methylcytosine dioxygenase activity
protein binding
DNA metabolism protein
nucleic acid metabolism protein
in utero embryonic development
neuron migration
placenta development
tRNA wobble cytosine modification
DNA repair
regulation of translation
regulation of translational initiation
regulation of translational elongation
cellular response to DNA damage stimulus
regulation of gene expression
cell differentiation
neuron projection development
oxidative RNA demethylation
RNA repair
negative regulation of neuron apoptotic process
developmental growth
positive chemotaxis
regulation of mitochondrial translation
positive regulation of gene expression, epigenetic
tRNA demethylation
DNA catabolic process, endonucleolytic
DNA dealkylation involved in DNA repair
oxidative demethylation
DNA demethylation
nucleic acid phosphodiester bond hydrolysis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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