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ABHD5 Polyclonal Antibody

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ABHD5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody 12201-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

ABHD5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

ABHD5 Antibody in Western Blot (WB) — Human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12201-1-AP (ABHD5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

ABHD5 Antibody in Immunoprecipitation (IP) — IP result of anti-ABHD5 (IP:12201-1-AP, 4ug; Detection:12201-1-AP 1:500) with Jurkat cells lysate 4000ug.

Product Details

12201-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Pig
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ABHD5 Fusion Protein Ag2840 (6-349 aa encoded by BC021958) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: EEVDSADTG ERSGWLTGWL PTWCPTSISH LKEAEEKMLK CVPCTYKKEP VRISNGNKIW TLKFSHNISN KTPLVLLHGF GGGLGLWALN FGDLCTNRPV YAFDLLGFGR SSRPRFDSDA EEVENQFVES IEEWRCALGL DKMILLGHNL GGFLAAAYSL KYPSRVNHLI LVEPWGFPER PDLADQDRPI PVWIRALGAA LTPFNPLAGL RIAGPFGLSL VQRLRPDFKR KYSSMFEDDT VTEYIYHCNV QTPSGETAFK NMTIPYGWAK RPMLQRIGKM HPDIPVSVIF GARSCIDGNS GTSIQSLRPH SYVKTIAILG AGHYVYADQP EEFNQKVKEI CDTVD (6-349 aa encoded by BC021958)

Target Information

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Maternal obesity aggravates the abnormality of porcine placenta by increasing N⁶-methyladenosine.

International journal of obesity (2005)

Song T,Lu J,Deng Z,Xu T,Yang Y,Wei H,Li S,Jiang S,Peng J

Mon Oct 01 00:00:00 EDT 2018

Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia.

eLife

Zhang X,Saarinen AM,Hitosugi T,Wang Z,Wang L,Ho TH,Liu J

Tue Dec 19 00:00:00 EST 2017

Regulation of G0/G1 Switch Gene 2 (G0S2) Protein Ubiquitination and Stability by Triglyceride Accumulation and ATGL Interaction.

PloS one

Heckmann BL,Zhang X,Saarinen AM,Liu J

Tue Jul 18 00:00:00 EDT 2017

CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development.

Journal of lipid research

Qi Y,Kapterian TS,Du X,Ma Q,Fei W,Zhang Y,Huang X,Dawes IW,Yang H

Sun May 01 00:00:00 EDT 2016

Regulation of FSP27 protein stability by AMPK and HSC70.

American journal of physiology. Endocrinology and metabolism

Zhang X,Heckmann BL,Xie X,Saarinen AM,Liu J

Mon Dec 01 00:00:00 EST 2014

Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.

Proceedings of the National Academy of Sciences of the United States of America

Patel S,Yang W,Kozusko K,Saudek V,Savage DB

Tue Jun 24 00:00:00 EDT 2014

Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.

Proceedings of the National Academy of Sciences of the United States of America

Patel S,Yang W,Kozusko K,Saudek V,Savage DB

Tue Jun 24 00:00:00 EDT 2014

Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.

Proceedings of the National Academy of Sciences of the United States of America

Patel S,Yang W,Kozusko K,Saudek V,Savage DB

Tue Jun 24 00:00:00 EDT 2014

Pleiotropic effects of cavin-1 deficiency on lipid metabolism.

The Journal of biological chemistry

Ding SY,Lee MJ,Summer R,Liu L,Fried SK,Pilch PF

Fri Mar 21 00:00:00 EDT 2014

Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.

Proceedings of the National Academy of Sciences of the United States of America

Olzmann JA,Richter CM,Kopito RR

Tue Jan 22 00:00:00 EST 2013

Distinct mechanisms regulate ATGL-mediated adipocyte lipolysis by lipid droplet coat proteins.

Molecular endocrinology (Baltimore, Md.)

Yang X,Heckmann BL,Zhang X,Smas CM,Liu J

Tue Jan 01 00:00:00 EST 2013

Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-α (TNF-α)-induced lipolysis in adipocytes.

The Journal of biological chemistry

Yang X,Zhang X,Heckmann BL,Lu X,Liu J

Fri Nov 25 00:00:00 EST 2011

Functional interactions between Mldp (LSDP5) and Abhd5 in the control of intracellular lipid accumulation.

The Journal of biological chemistry

Granneman JG,Moore HH,Mottillo EP,Zhu Z

Fri Jan 30 00:00:00 EST 2009

Bioinformatics

Protein Aliases: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; Abhydrolase domain-containing protein 5; aCGI58; CGI 58; CGI58 protein; Lipid droplet-binding protein CGI-58; MGC8731; Protein CGI-58; truncated abhydrolase domain-containing protein 5

Gene Aliases: 1300003D03Rik; 2010002J10Rik; ABHD5; CDS; CGI-58; CGI58; IECN2; IECN5; NCIE2

UniProt ID: (Human) Q8WTS1, (Mouse) Q9DBL9

Entrez Gene ID: (Human) 51099, (Mouse) 67469

Function(s)

lipid metabolic process fatty acid metabolic process phosphatidic acid biosynthetic process phospholipid biosynthetic process negative regulation of sequestering of triglyceride positive regulation of triglyceride catabolic process cell differentiation positive regulation of lipoprotein lipase activity

Process(es)

1-acylglycerol-3-phosphate O-acyltransferase activity triglyceride lipase activity lysophosphatidic acid acyltransferase activity carboxylic ester hydrolase activity protein binding transferase activity transferase activity, transferring acyl groups