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Invitrogen
ABHD5 Polyclonal Antibody
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FIGURE: 1 / 9
ABHD5 Antibody (PA5-78704) in ICC/IF
Immunocytochemistry analysis of Abhd5 using anti-Abhd5 antibody (Product # PA5-78704) . Abhd5 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Abhd5 antibody (Product # PA5-78704) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. V... View More
Product Details
PA5-78704
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Abhd5 recombinant protein (Position: R169-D349).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, A431 whole cell. IHC: human intestinal cancer tissue. ICC/IF: A431 cell.
Target Information
The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; Abhydrolase domain-containing protein 5; aCGI58; CGI 58; CGI58 protein; Lipid droplet-binding protein CGI-58; MGC8731; Protein CGI-58; unnamed protein product
Gene Aliases: 1300003D03Rik; 2010002J10Rik; ABHD5; CDS; CGI-58; CGI58; IECN2; IECN5; NCIE2
UniProt ID: (Human) Q8WTS1, (Mouse) Q9DBL9, (Rat) Q6QA69
Entrez Gene ID: (Human) 51099, (Mouse) 67469, (Rat) 316122
lipid metabolic process
fatty acid metabolic process
phosphatidic acid biosynthetic process
phospholipid biosynthetic process
negative regulation of sequestering of triglyceride
positive regulation of triglyceride catabolic process
cell differentiation
positive regulation of lipoprotein lipase activity
positive regulation of lipid catabolic process
lipid homeostasis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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