Invitrogen

ABHEB Polyclonal Antibody

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ABHEB Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of ABHEB in human cell line U-2 OS shows positivity in nucleus, nucleoli & cytoplasm. Samples were probed using an ABHEB Polyclonal Antibody (Product # PA5-57661).

ABHEB Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ABHEB in human pancreas shows strong cytoplasmic and nuclear positivity in exocrine glandular cells and islet cells. Samples were probed using an ABHEB Polyclonal Antibody (Product # PA5-57661).

ABHEB Antibody in Western Blot (WB) — Western blot analysis of ABHEB in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ABHEB Polyclonal Antibody (Product # PA5-57661).

Product Details

PA5-57661

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human ABHEB View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2637564

Product Specific Information

Immunogen sequence: PICTDKINAA NYASVKTPAL IVYGDQDPMG QTSFEHLKQL PNHRVLIMKG AGHPCYLDKP EEWHTGLLDF L

Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 87%.

Target Information

ABHEB has hydrolase activity towards p-nitrophenyl butyrate (in vitro). It may activate transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Abhydrolase domain-containing protein 14B; Alpha/beta hydrolase domain-containing protein 14B; CCG1-interacting factor B; cell cycle gene 1-interacting factor B; epididymis secretory protein Li 299; Protein ABHD14B

Gene Aliases: ABHD14B; CIB; HEL-S-299

UniProt ID: (Human) Q96IU4

Entrez Gene ID: (Human) 84836

Function(s)

positive regulation of transcription from RNA polymerase II promoter 3'-phosphoadenosine 5'-phosphosulfate metabolic process

Process(es)

protein binding hydrolase activity