Invitrogen

ACADL Polyclonal Antibody

View all (11) ACADL antibodies

Questions & Answers

Questions & Answers

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ACADL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ACADL in paraffin-embedded human kidney tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with ACADL polyclonal antibody (Product # PA5-109332) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

ACADL Antibody in Western Blot (WB) — Western blot analysis of ACADL in SH-SY5Y cell lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with ACADL polyclonal antibody (Product # PA5-109332), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.

ACADL Antibody in Flow Cytometry (Flow) — Flow cytometry of ACADL in F9 cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with ACADL polyclonal antibody (Product # PA5-109332) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:1000 (30 min).

Product Details

PA5-109332

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein within Human ACADL aa 218-430 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2854743

Product Specific Information

Positive Control: SH-SY5Y cell, human kidney tissue, F9.

Target Information

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acyl-Coenzyme A dehydrogenase, long chain; LCAD; Long-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene Aliases: ACAD4; ACADL; LCAD

UniProt ID: (Human) P28330

Entrez Gene ID: (Human) 33

Molecular Function: dehydrogenase

temperature homeostasis fatty acid beta-oxidation carnitine metabolic process, CoA-linked fatty acid beta-oxidation using acyl-CoA dehydrogenase carnitine catabolic process long-chain fatty acid catabolic process cellular lipid catabolic process negative regulation of fatty acid biosynthetic process negative regulation of fatty acid oxidation protein homotetramerization lipid homeostasis oxidation-reduction process regulation of cholesterol metabolic process

Process(es)

fatty-acyl-CoA binding acyl-CoA dehydrogenase activity long-chain-acyl-CoA dehydrogenase activity electron carrier activity palmitoyl-CoA oxidase activity flavin adenine dinucleotide binding oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor