Invitrogen

ACADS Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (10) ACADS antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ACADS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of ACADS in human cell line Hep G2 using ACADS Polyclonal Antibody (Product # PA5-54580) shows localization to mitochondria.

ACADS Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ACADS in human liver using an ACADS Polyclonal Antibody (Product # PA5-54580) shows strong cytoplasmic positivity with a granular pattern in hepatocytes and bile duct cells.

ACADS Antibody in Western Blot (WB) — Western blot analysis of ACADS in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).

ACADS Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ACADS demonstrates antibody specificity. Immunohistochemical analysis of ACADS using anti-ACADS Polyclonal Antibody (Product # PA5-54580), shows significant staining of ACADS in human duodenum and shows minimal or weak staining in human tonsil tissues. The relative expression levels of ACADS within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-54580

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human ACADS View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2637583

Product Specific Information

Immunogen sequence: ELPETHQMLL QTCRDFAEKE LFPIAAQVDK EHLFPAAQVK KMGGLGLLAM DVPEELGGAG LDYLAYAIAM EEISRGCAST GVIMSVNNSL YLGPILKFGS KEQKQAWVTP FTSGDKIGCF ALSEPGNGS

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 89%.

Target Information

Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i. e. less than 6 carbon atoms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation.

Cell communication and signaling : CCS

Hao YH,Lafita-Navarro MC,Zacharias L,Borenstein-Auerbach N,Kim M,Barnes S,Kim J,Shay J,DeBerardinis RJ,Conacci-Sorrell M

PA5-54580 was used in Western Blotting to show myc regulates proliferation through activation of LEF1 expression and the WNT patway and that it is a transcriptional regulator of LEF1 in colonic cells.

Thu Oct 17 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: acetyl-Coenzyme A dehydrogenase, short chain; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; acyl-Coenzyme A dehydrogenase, short chain; Butyryl-CoA dehydrogenase; mitochondrial short-chain specific acyl-CoA dehydrogenase; SCAD; short chain acyl-coenzyme A dehydrogenase; short-chain acyl-CoA dehydrogenase; Short-chain specific acyl-CoA dehydrogenase, mitochondrial; unsaturated acyl-CoA reductase

Gene Aliases: ACAD3; ACADS; AI196007; Bcd-1; Bcd1; Hdlq8; SCAD

UniProt ID: (Human) P16219, (Rat) P15651, (Mouse) Q07417

Entrez Gene ID: (Human) 35, (Rat) 64304, (Mouse) 11409

Molecular Function: dehydrogenase