Invitrogen

ACADS Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (8) ACADS antibodies

Datasheet

Datasheet

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ACADS Antibody in Immunofluorescence (IF) — Immunofluorescent staining of ACADS in human cell line Hep G2 using ACADS Polyclonal Antibody (Product # PA5-54580) shows localization to mitochondria.

ACADS Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ACADS in human liver using an ACADS Polyclonal Antibody (Product # PA5-54580) shows strong cytoplasmic positivity with a granular pattern in hepatocytes and bile duct cells.

ACADS Antibody in Western Blot (WB) — Western blot analysis of ACADS in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).

ACADS Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of ACADS demonstrates antibody specificity. Immunohistochemical analysis of ACADS using anti-ACADS Polyclonal Antibody (Product # PA5-54580), shows significant staining of ACADS in human duodenum and shows minimal or weak staining in human tonsil tissues. The relative expression levels of ACADS within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/ml

Immunofluorescence (IF)

1-4 µg/ml

Immunohistochemistry (IHC)

1:500-1:1000

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ACADS

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2637583

Product Specific Information

Immunogen sequence: ELPETHQMLL QTCRDFAEKE LFPIAAQVDK EHLFPAAQVK KMGGLGLLAM DVPEELGGAG LDYLAYAIAM EEISRGCAST GVIMSVNNSL YLGPILKFGS KEQKQAWVTP FTSGDKIGCF ALSEPGNGS

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 89%.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ACAD3; acetyl-Coenzyme A dehydrogenase, short chain; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; acyl-Coenzyme A dehydrogenase, short chain; Butyryl-CoA dehydrogenase; mitochondrial short-chain specific acyl-CoA dehydrogenase; SCAD; short chain acyl-coenzyme A dehydrogenase; short-chain acyl-CoA dehydrogenase; Short-chain specific acyl-CoA dehydrogenase, mitochondrial; unsaturated acyl-CoA reductase

Gene Aliases: ACAD3; ACADS; AI196007; Bcd-1; Bcd1; Hdlq8; SCAD

UniProt ID: (Human) P16219, (Rat) P15651, (Mouse) Q07417

Entrez Gene ID: (Human) 35, (Rat) 64304, (Mouse) 11409

Molecular Function: dehydrogenase oxidase oxidoreductase transferase