Invitrogen

ACAT1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (14) ACAT1 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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ACAT1 Antibody in Western Blot (WB) — Knockdown of ACAT1 was achieved by transfecting HepG2 cells with ACAT1 specific siRNAs (Silencer® select Product # s901). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the ACAT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ACAT1 Polyclonal Antibody (Product # PA5-19227, 0.2µg/ml) and Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ACAT1.

ACAT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HepG2 cells were transfected with ACAT1 siRNA and reduction of signal was observed in Western Blot using ACAT1 Polyclonal Antibody (Product # PA5-19227). {KD}

Product Details

PA5-19227

Applications	Tested Dilution	Publications
Western Blot (WB)	0.01-0.03 µg/mL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (DEEYKRVDFSKVPK) corresponding to the internal amino acids of ACAT1
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10977900

Product Specific Information

This antibody is predicted to react with bovine and canine based on sequence homology.

Target Information

ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pimozide Alleviates Hyperglycemia in Diet-Induced Obesity by Inhibiting Skeletal Muscle Ketone Oxidation.

Cell metabolism

Al Batran R,Gopal K,Capozzi ME,Chahade JJ,Saleme B,Tabatabaei-Dakhili SA,Greenwell AA,Niu J,Almutairi M,Byrne NJ,Masson G,Kim R,Eaton F,Mulvihill EE,Garneau L,Masters AR,Desta Z,Velázquez-Martínez CA,Aguer C,Crawford PA,Sutendra G,Campbell JE,Dyck JRB,Ussher JR

PA5-19227 was used in Western Blotting to report that activity of the rate-limiting enzyme for ketone body oxidation, succinyl-CoA:3-ketoacid-CoA transferase (SCOT/Oxct1), is increased in muscles of obese mice.

Tue May 05 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; acetyl-Co A acetyltransferase 1 mitochondrial; Acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; T2; testicular tissue protein Li 198

Gene Aliases: 6330585C21Rik; ACAT; ACAT1; MAT; RATACAL; T2; THIL

UniProt ID: (Human) P24752, (Rat) P17764, (Mouse) Q8QZT1

Entrez Gene ID: (Human) 38, (Rat) 25014, (Mouse) 110446

Molecular Function: acyltransferase