Invitrogen

ACAT1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (13) ACAT1 antibodies

Datasheet

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Datasheet

Questions & Answers

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ACAT1 Antibody in Western Blot (WB) — Knockdown of ACAT1 was achieved by transfecting HepG2 cells with ACAT1 specific siRNAs (Silencer® select Product # s901). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the ACAT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ACAT1 Polyclonal Antibody (Product # PA5-19227, 0.2µg/ml) and Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ACAT1.

ACAT1 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HepG2 cells were transfected with ACAT1 siRNA and reduction of signal was observed in Western Blot using ACAT1 Polyclonal Antibody (Product # PA5-19227). {KD}

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

Assay Dependent

Western Blot (WB)

0.01-0.03 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DEEYKRVDFSKVPK) corresponding to the internal amino acids of ACAT1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10977900

Product Specific Information

This antibody is predicted to react with bovine and canine based on sequence homology.

Target Information

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ACAT1; acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; acetyl-Co A acetyltransferase 1 mitochondrial; Acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; T2; testicular tissue protein Li 198; THIL

Gene Aliases: 6330585C21Rik; ACAT; ACAT1; MAT; RATACAL; T2; THIL

UniProt ID: (Human) P24752, (Rat) P17764, (Mouse) Q8QZT1

Entrez Gene ID: (Human) 38, (Rat) 25014, (Mouse) 110446

Molecular Function: acetyltransferase transferase