Immunofluorescent analysis of ACAT1 showing staining in the mitochondria of A431 cells. A431 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using an ACAT1 polyclonal antibody (Product # PA5-34676) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 151 of ACAT1 (Uniprot ID#P24752)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34676 targets ACAT1 in WB applications and shows reactivity with Human and Mouse samples.
The PA5-34676 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 151 of ACAT1 (Uniprot ID#P24752).
ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.