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Invitrogen
ACBD3 Polyclonal Antibody
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FIGURE: 1 / 4
ACBD3 Antibody (PA5-113338) in ICC/IF
Immunocytochemichal analysis of ACBD3 in Hela cells using a Polyclonal antibody (Product # PA5-113338) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-113338
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Golgi resident protein GCP60 protein (263-366AA)
Conjugate
Form
Liquid
Concentration
2.8 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acyl-CoA-binding domain-containing protein 3; acyl-Coenzyme A binding domain containing 3; DAP; DMT1-associated protein; GOCAP1; golgi complex associated protein 1, 60kDa; Golgi complex-associated protein 1; Golgi phosphoprotein 1; Golgi resident protein GCP60; GOLPH1; neurotrophin receptor alike death domain protein; p75-like apoptosis-inducing death domain protein; PBR- and PKA-associated protein 7; Peripheral benzodiazepine receptor-associated protein PAP7; PKA (RIalpha)-associated protein
Gene Aliases: ACBD3; GCP60; GOCAP1; GOLPH1; PAP7
UniProt ID: (Human) Q9H3P7, (Rat) Q7TNY6
Entrez Gene ID: (Human) 64746, (Rat) 289312
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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