Invitrogen

ACBD5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (9) ACBD5 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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ACBD5 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of ACBD5 in human cell line A-431 using an ACBD5 Polyclonal Antibody (Product # PA5-52861) shows localization to peroxisomes.

ACBD5 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ACBD5 in human adrenal gland, colon, liver and testis using ACBD5 Polyclonal Antibody (Product # PA5-52861) (A) shows similar protein distribution across tissues to an independent ACBD5 Polyclonal Antibody (B).

ACBD5 Antibody in Western Blot (WB) — Western blot analysis of ACBD5 in human cell lines A-549 and PC-3 using a ACBD5 Polyclonal Antibody (Product # PA5-52861). Corresponding ACBD5 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

ACBD5 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of ACBD5 demonstrates antibody specificity. Immunohistochemical analysis of ACBD5 using anti-ACBD5 Polyclonal Antibody (Product # PA5-52861), shows significant staining of ACBD5 in human parathyroid gland and shows minimal or weak staining in human human pancreas tissues. The relative expression levels of ACBD5 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ACBD5

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2637589

Product Specific Information

Immunogen sequence: IQVPPGNGNI GNMQVVAVEG KGEVKHGGED GRNNSGAPHR EKRGGETDEF SNVRRGRGHR MQHLSEGTKG RQVGSGGDGE RWGSDRGSRG SLNEQIALVL MRLQEDMQNV LQRLQKLETL TALQAKSSTS TLQTAPQPTS QRPSWWPF

Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 81%.

Target Information

Acyl-CoA binding protein which acts as the peroxisome receptor for pexophagy but is dispensable for aggrephagy and nonselective autophagy. Binds medium- and long-chain acyl-CoA esters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: Acyl-CoA-binding domain-containing protein 5; acyl-Coenzyme A binding domain containing 5; endozepine-related protein; membrane-associated diazepam binding inhibitor

Gene Aliases: ACBD5; KIAA1996

UniProt ID: (Human) Q5T8D3

Entrez Gene ID: (Human) 91452

Molecular Function: enzyme modulator transfer/carrier protein