Invitrogen

ACER3 Polyclonal Antibody

View all (3) ACER3 antibodies

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Protocols

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ACER3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ACER3 in COLO205 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ACER3 polyclonal antibody (Product # PA5-101416) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

ACER3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ACER3 in human breast cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ACER3 polyclonal antibody (Product # PA5-101416) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ACER3 Antibody in Western Blot (WB) — Western blot analysis of ACER3 in COLO cells. Samples were incubated with ACER3 polyclonal antibody (Product # PA5-101416).

Product Details

PA5-101416

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ACER3(Accession Q9NUN7), corresponding to amino acid residues L187-Q215. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2850852

Target Information

ACER3 is a protein coding gene. Endoplasmic reticulum and Golgi ceramidase that catalyzes the hydrolysis of unsaturated long-chain C18:1-, C20:1- and C20:4-ceramides, dihydroceramides and phytoceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Controls the generation of sphingosine in erythrocytes, and thereby sphingosine-1-phosphate in plasma. Through the regulation of ceramides and sphingosine-1-phosphate homeostasis in the brain may play a role in neurons survival and function. By regulating the levels of proinflammatory ceramides in immune cells and tissues, may modulate the inflammatory response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alkaline CDase 3; Alkaline ceramidase 3; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; AlkCDase 3; aPHC; phytoceramidase, alkaline

Gene Aliases: 1110057L18Rik; 5430429L08Rik; ACER3; APHC; AV015045; PHCA; RGD1561254

UniProt ID: (Human) Q9NUN7, (Mouse) Q9D099

Entrez Gene ID: (Human) 55331, (Mouse) 66190, (Rat) 499210

Function(s)

ceramide metabolic process positive regulation of cell proliferation sphingosine biosynthetic process phytosphingosine biosynthetic process biological_process sphingolipid biosynthetic process

Process(es)

phytoceramidase activity histone deacetylase activity protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity N-acetylgalactosamine-6-phosphate deacetylase activity diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity molecular_function

ACER3 Polyclonal Antibody

Product Details

PA5-101416

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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