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ACK1 Antibody (PA5-96151) in IHC (P)
Immunohistochemistry analysis of ACK1 in paraffin-embedded mouse kidney. Samples were incubated with ACK1 Polyclonal antibody (Product # PA5-96151) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-96151
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 920-1038 of human TNK2 (NP_0057723)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.32 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807953
Product Specific Information
Immunogen sequence: KANFSTNNSN PGARPPPPRA TARLPQRGCP GDGPEAGRPA DKIQMAMVHG VTTEECQAAL QCHGWSVQRA AQYLKVEQLF GLGLRPRGEC HKVLEMFDWN LEQAGCHLLG SWGPAHHKR
Positive Samples: SW620, HT-29, MCF7, Raji; Cellular Location: Cell junction, Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endosome, Membrane, Nucleus, Peripheral membrane protein, adherens junction, clathrin-coated pit, clathrin-coated vesicle
Target Information
TNK2 (ACK) (activated Cdc42-associated kinase) is a non-receptor tyrosine kinase that signals downstream of Cdc42. TNK2 is known to be involved in tumorigenesis and metastasis, particularly in prostate cancer. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACK-1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; Cdc42 GTPase-inhibiting protein; Non-receptor protein tyrosine kinase Ack; P21cdc42Hs kinase; tnk2 {ECO:0000250|UniProtKB:Q07912}; Tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2
Gene Aliases: ACK; ACK-1; ACK1; Cdgip; p21cdc42Hs; Pyk1; TNK2
UniProt ID: (Human) Q07912, (Mouse) O54967, (Rat) Q5U2X5
Entrez Gene ID: (Human) 10188, (Mouse) 51789, (Rat) 303882
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
GTPase inhibitor activity
epidermal growth factor receptor binding
protein binding
ATP binding
identical protein binding
metal ion binding
WW domain binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
hormone receptor binding
protein phosphorylation
endocytosis
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
cell differentiation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
innate immune response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of clathrin-mediated endocytosis
cell surface receptor signaling pathway
small GTPase mediated signal transduction
negative regulation of GTPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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