Invitrogen

ACSL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 References

View all (11) ACSL1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ACSL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ACSL1 was performed using 70% confluent log phase MCF-10A and MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ACSL1 Polyclonal Antibody (Product # PA5-17136) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondria like staining of ACSL1 in MCF-10A and not in MCF7 (panel e) which is reported negative for the same. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ACSL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ACSL1 in paraffin-embedded human lung carcinoma using an ACSL1 polyclonal antibody (Product # PA5-17136).

ACSL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-ACSL1 Polyclonal Antibody (Product # PA5-17136) and a 77 kDa band corresponding to ACSL1 was observed across cell lines and tissues tested except in MCF7, undifferentiated 3T3-L1 and Mouse Spleen, which are reported to be negative for ACSL1. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF-10A (Lane 2), 3T3-L1 (Lane 3), 3T3-L1 differentiated to adipocytes (Lane 4), tissue extracts of Mouse Adipose (Lane 5), Mouse Spleen (Lane 6), Mouse Kidney (Lane 7), Mouse Liver (Lane 8), Rat Kidney (Lane 9) and Rat Adipose (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

ACSL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of ACSL1 was observed in MCF-10A in comparison to MCF7 in cell lines and in Mouse Adipose, Mouse Liver in comparison to Mouse Spleen and Mouse Kidney which are reported to be low expressing for ACSL1 using Anti-ACSL1 Polyclonal Antibody (Product # PA5-17136) in Western Blot. (doi:10.1093/carcin/bgx020; 10.1038/srep19661 (2016)). {RE}

Product Details

PA5-17136

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a sequence of human ACSL1
Conjugate	Unconjugated
Form	Liquid
Concentration	36 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	no preservative
Storage conditions	-20°C
RRID	AB_10984144

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomic and Structural Manifestations of Cardiomyopathy in Rat Models of Obesity and Weight Loss.

Frontiers in endocrinology

Liśkiewicz AD,Marczak Ł,Bogus K,Liśkiewicz D,Przybyła M,Lewin-Kowalik J

PA5-17136 was used in Western Blotting to characterize proteomic and structural changes in cardiomyopathy.

Tue Dec 21 00:00:00 EST 2021

Ca2+ Binding/Permeation via Calcium Channel, CaV1.1, Regulates the Intracellular Distribution of the Fatty Acid Transport Protein, CD36, and Fatty Acid Metabolism.

The Journal of biological chemistry

Georgiou DK,Dagnino-Acosta A,Lee CS,Griffin DM,Wang H,Lagor WR,Pautler RG,Dirksen RT,Hamilton SL

Published figure using ACSL1 polyclonal antibody (Product # PA5-17136) in Miscellaneous

Fri Sep 25 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: ACS1; Acyl CoA synthetase, long chain; Acyl-CoA synthetase 1; Arachidonate--CoA ligase; fatty acid Coenzyme A ligase, long chain 2; fatty-acid-Coenzyme A ligase, long-chain 1; fatty-acid-Coenzyme A ligase, long-chain 2; L LACS2; LACS 1; LACS 2; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 1; Long-chain acyl-CoA synthetase 2; Long-chain fatty acid-CoA ligase 2; long-chain fatty-acid-coenzyme A ligase 1; Long-chain-fatty-acid--CoA ligase 1; Long-chain-fatty-acid--CoA ligase, liver isozyme; OTTHUMP00000218457; OTTHUMP00000218460; OTTHUMP00000218462; OTTHUMP00000218465; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; paltimoyl-CoA ligase 1; Phytanate--CoA ligase

Gene Aliases: Acas; Acas1; ACS; ACS1; ACSL1; Acsl2; COAA; FACL1; FACL2; FACS; LACS; LACS 1; LACS1; LACS2

UniProt ID: (Human) P33121, (Mouse) P41216, (Rat) P18163

Entrez Gene ID: (Human) 2180, (Mouse) 14081, (Rat) 25288

Molecular Function: ligase