Proteintech

ACTN2 Polyclonal Antibody

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ACTN2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACTN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ACTN2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using (Product # 14221-1-AP) (ACTN2 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ACTN2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ACTN2 Antibody in Immunoprecipitation (IP) — IP result of anti-ACTN2 (IP:14221-1-AP, 4ug; Detection:14221-1-AP 1:1000) with HeLa cells lysate 1080ug.

Product Details

14221-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ACTN2 Fusion Protein Ag5459 (1-301 aa encoded by BC051770)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MNQIEPGVQ YNYVYDEDEY MIQEEEWDRD LLLDPAWEKQ QRKTFTAWCN SHLRKAGTQI ENIEEDFRNG LKLMLLLEVI SGERLPKPDR GKMRFHKIAN VNKALDYIAS KGVKLVSIGA EEIVDGNVKM TLGMIWTIIL RFAIQDISVE ETSAKEGLLL WCQRKTAPYR NVNIQNFHTS WKDGLGLCAL IHRHRPDLID YSKLNKDDPI GNINLAMEIA EKHLDIPKML DAEDIVNTPK PDERAIMTYV SCFYHAFAGA EQAETAANRI CKVLAVNQEN ERLMEEYERL ASELLEWIRR TI (1-301 aa encoded by BC051770)

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hitachi K,Kiyofuji Y,Yamaguchi H,Nakatani M,Inui M,Tsuchida K

Sun Jan 01 00:00:00 EST 2023

Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hitachi K,Kiyofuji Y,Yamaguchi H,Nakatani M,Inui M,Tsuchida K

Sun Jan 01 00:00:00 EST 2023

A catechol bioadhesive for rapid hemostasis and healing of traumatic internal organs and major arteries.

Biomaterials

Wang G,Meng X,Wang P,Wang X,Liu G,Wang DA,Fan C

Thu Dec 01 00:00:00 EST 2022

Essential role of MESP1-RING1A complex in cardiac differentiation.

Developmental cell

Liang Q,Wang S,Zhou X,Li Y,Xing S,Sha Y,Yang F,Huang W,Liu N,Li Z,Chen Y,Xu Y,Zhu P,Lan F,Sun N

Mon Nov 21 00:00:00 EST 2022

Effects of macrophages on the proliferation and cardiac differentiation of human induced pluripotent stem cells.

Cell communication and signaling : CCS

Long C,Guo R,Han R,Li K,Wan Y,Xu J,Gong X,Zhao Y,Yao X,Liu J

Mon Jul 18 00:00:00 EDT 2022

Identification of differential microRNAs and messenger RNAs resulting from ASXL transcriptional regulator 3 knockdown during during heart development.

Bioengineered

Liu ZQ,Cheng M,Fu F,Li R,Han J,Yang X,Deng Q,Li LS,Lei TY,Li DZ,Liao C

Fri Apr 01 00:00:00 EDT 2022

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Basic research in cardiology

Hulikova A,Park KC,Loonat AA,Gunadasa-Rohling M,Curtis MK,Chung YJ,Wilson A,Carr CA,Trafford AW,Fournier M,Moshnikova A,Andreev OA,Reshetnyak YK,Riley PR,Smart N,Milne TA,Crump NT,Swietach P

Thu Mar 31 00:00:00 EDT 2022

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Nature communications

Qian W,Hadi T,Silvestro M,Ma X,Rivera CF,Bajpai A,Li R,Zhang Z,Qu H,Tellaoui RS,Corsica A,Zias AL,Garg K,Maldonado T,Ramkhelawon B,Chen W

Wed Jan 26 00:00:00 EST 2022

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Nature communications

Qian W,Hadi T,Silvestro M,Ma X,Rivera CF,Bajpai A,Li R,Zhang Z,Qu H,Tellaoui RS,Corsica A,Zias AL,Garg K,Maldonado T,Ramkhelawon B,Chen W

Wed Jan 26 00:00:00 EST 2022

Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Frontiers in cell and developmental biology

Gilbert G,Kadur Nagaraju C,Duelen R,Amoni M,Bobin P,Eschenhagen T,Roderick HL,Sampaolesi M,Sipido KR

Tue Nov 23 00:00:00 EST 2021

Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Frontiers in cell and developmental biology

Gilbert G,Kadur Nagaraju C,Duelen R,Amoni M,Bobin P,Eschenhagen T,Roderick HL,Sampaolesi M,Sipido KR

Tue Nov 23 00:00:00 EST 2021

Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy.

Frontiers in cell and developmental biology

Li B,Xiong W,Liang WM,Chiou JS,Lin YJ,Chang ACY

Wed Apr 21 00:00:00 EDT 2021

A ceRNA-associated risk model predicts the poor prognosis for head and neck squamous cell carcinoma patients.

Scientific reports

Xu Y,Xu F,Lv Y,Wang S,Li J,Zhou C,Jiang J,Xie B,He F

Thu Mar 18 00:00:00 EDT 2021

Mutation location of HCM-causing troponin T mutations defines the degree of myofilament dysfunction in human cardiomyocytes.

Journal of molecular and cellular cardiology

Schuldt M,Johnston JR,He H,Huurman R,Pei J,Harakalova M,Poggesi C,Michels M,Kuster DWD,Pinto JR,van der Velden J

Fri Jan 01 00:00:00 EST 2021

LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis.

Journal of molecular and cellular cardiology

Xu Y,Luo Y,Liang C,Zhang T

Sat Feb 01 00:00:00 EST 2020

LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis.

Journal of molecular and cellular cardiology

Xu Y,Luo Y,Liang C,Zhang T

Sat Feb 01 00:00:00 EST 2020

Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.

Progress in biophysics and molecular biology

He X,Li L,Tang M,Zeng Y,Li H,Yu X

Fri Nov 01 00:00:00 EDT 2019

Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.

Progress in biophysics and molecular biology

He X,Li L,Tang M,Zeng Y,Li H,Yu X

Fri Nov 01 00:00:00 EDT 2019

MiR-466h-5p induces expression of myocardin with complementary promoter sequences.

Biochemical and biophysical research communications

Luo Y,Liang C,Xu Y,Zhang T

Tue Jun 18 00:00:00 EDT 2019

Possible mechanism of GATA4 inhibiting myocardin activity during cardiac hypertrophy.

Journal of cellular biochemistry

Xu Y,Liang C,Luo Y,Xing W,Zhang T

Sat Jun 01 00:00:00 EDT 2019

Early Decline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Doklady. Biochemistry and biophysics

Petrova IO,Tyganov SA,Mirzoev TM,Tsaturyan AK,Kozlovskaya IB,Shenkman BS

Sun Jul 01 00:00:00 EDT 2018

Early Decline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Doklady. Biochemistry and biophysics

Petrova IO,Tyganov SA,Mirzoev TM,Tsaturyan AK,Kozlovskaya IB,Shenkman BS

Sun Jul 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: actinin, alpha 2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein

Gene Aliases: 1110008F24Rik; ACTN2; CMD1AA; CMH23

UniProt ID: (Human) P35609, (Mouse) Q9JI91

Entrez Gene ID: (Human) 88, (Mouse) 11472, (Rat) 291245

Function(s)