Proteintech

ACTN2 Polyclonal Antibody

Product Image Gallery

ACTN2 Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

ACTN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ACTN2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACTN2 Antibody in Immunoprecipitation (IP) — IP result of anti-ACTN2 (IP:14221-1-AP, 4ug; Detection:14221-1-AP 1:1000) with HeLa cells lysate 1080ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1000

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ACTN2 Fusion Protein Ag5459

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: actinin, alpha 2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein

Gene Aliases: 1110008F24Rik; ACTN2; CMD1AA; CMH23

UniProt ID: (Human) P35609, (Mouse) Q9JI91

Entrez Gene ID: (Human) 88, (Mouse) 11472, (Rat) 291245

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein