Proteintech

ACTN2 Polyclonal Antibody

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ACTN2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACTN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ACTN2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACTN2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ACTN2 Antibody in Immunoprecipitation (IP) — IP result of anti-ACTN2 (IP:14221-1-AP, 4ug; Detection:14221-1-AP 1:1000) with HeLa cells lysate 1080ug.

Product Details

14221-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:250-1:1,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ACTN2 Fusion Protein Ag5459 (1-301 aa encoded by BC051770) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MNQIEPGVQ YNYVYDEDEY MIQEEEWDRD LLLDPAWEKQ QRKTFTAWCN SHLRKAGTQI ENIEEDFRNG LKLMLLLEVI SGERLPKPDR GKMRFHKIAN VNKALDYIAS KGVKLVSIGA EEIVDGNVKM TLGMIWTIIL RFAIQDISVE ETSAKEGLLL WCQRKTAPYR NVNIQNFHTS WKDGLGLCAL IHRHRPDLID YSKLNKDDPI GNINLAMEIA EKHLDIPKML DAEDIVNTPK PDERAIMTYV SCFYHAFAGA EQAETAANRI CKVLAVNQEN ERLMEEYERL ASELLEWIRR TI (1-301 aa encoded by BC051770)

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.

Progress in biophysics and molecular biology

He X,Li L,Tang M,Zeng Y,Li H,Yu X

Fri Nov 01 00:00:00 EDT 2019

Possible mechanism of GATA4 inhibiting myocardin activity during cardiac hypertrophy.

Journal of cellular biochemistry

Xu Y,Liang C,Luo Y,Xing W,Zhang T

Sat Jun 01 00:00:00 EDT 2019

Early Decline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Doklady. Biochemistry and biophysics

Petrova IO,Tyganov SA,Mirzoev TM,Tsaturyan AK,Kozlovskaya IB,Shenkman BS

Sun Jul 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: actinin, alpha 2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein

Gene Aliases: 1110008F24Rik; ACTN2; CMD1AA; CMH23

UniProt ID: (Human) P35609, (Mouse) Q9JI91

Entrez Gene ID: (Human) 88, (Mouse) 11472, (Rat) 291245

Function(s)