Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:250-1:1,000 | - |
Immunohistochemistry (PFA fixed) (IHC (PFA)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ACTN2 Fusion Protein Ag5459 (1-301 aa encoded by BC051770) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.19 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MNQIEPGVQ YNYVYDEDEY MIQEEEWDRD LLLDPAWEKQ QRKTFTAWCN SHLRKAGTQI ENIEEDFRNG LKLMLLLEVI SGERLPKPDR GKMRFHKIAN VNKALDYIAS KGVKLVSIGA EEIVDGNVKM TLGMIWTIIL RFAIQDISVE ETSAKEGLLL WCQRKTAPYR NVNIQNFHTS WKDGLGLCAL IHRHRPDLID YSKLNKDDPI GNINLAMEIA EKHLDIPKML DAEDIVNTPK PDERAIMTYV SCFYHAFAGA EQAETAANRI CKVLAVNQEN ERLMEEYERL ASELLEWIRR TI (1-301 aa encoded by BC051770)
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actinin, alpha 2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein
Gene Aliases: 1110008F24Rik; ACTN2; CMD1AA; CMH23
UniProt ID: (Human) P35609, (Mouse) Q9JI91
Entrez Gene ID: (Human) 88, (Mouse) 11472, (Rat) 291245
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