|Tested species reactivity||Bovine, Human, Pig|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C K(636) N G Y Q S D A K G V K T F(649) of human ADAMTS5.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-1751A detects ADAMTS5 from porcine, bovine, and human samples. PA1-1751A is specific for ADAMTS5 and shows no cross-reactivity with other ADAMTS family members.
PA1-1751A has been successfully used in Western blot procedures. By Western blot, this antibody detects a major band at ~68 kDa protein representing ADAMTS5 from porcine cartilage extract. On human recombinant ADAMTS5 this antibody detects a major band at ~85 kDa. Depending on the extent of C-terminal processing, several smaller bands may also be seen using this antibody.
PA1-1751A immunogen is a synthetic peptide corresponding to residues C K(636) N G Y Q S D A K G V K T F(649) of human ADAMTS5. This polyclonal antibody has been referred to as: JSCKNG.
A disintegrin-like and metalloproteinase thrombospondin type 1 motif 5 (ADAMTS5 or aggrecanase 2) is a member of the ADAMTS family of multidomain proteins that has demonstrated metalloproteinase functions. The ADAMTS5 protein contains a prohormone convertase cleavage site, thrombospondin-1 like domain, a zinc-binding motif, a disintegrin-like domain, cysteine-rich domain, and a propeptide domain. ADAMTS5 expression is found in a wide range of human tissues, including the placenta, bladder, rib cartilage, cervix and joint tissue.
ADAMTS5 plays an important role in the cleavage of aggrecan and studies have determined that aggrecan is cleaved within the interglobular domain of aggrecan at site Glu(373)-Ala(374). Aggrecan degradation has been linked to the development of arthritic diseases such as rheumatoid arthritis and osteoarthritis as well as retinal disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Synthesis and organization of hyaluronan and versican by embryonic stem cells undergoing embryoid body differentiation.
PA1-1751A was used in immunohistochemistry to investigate hyaluronan and versican during embryonic stem differentiation
|Shukla S,Nair R,Rolle MW,Braun KR,Chan CK,Johnson PY,Wight TN,McDevitt TC||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (58:345)||2010|