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Invitrogen
ADAR Polyclonal Antibody
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Product Details
PA5-49815
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from C-terminal of human ADAR1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total ADAR protein.
Target Information
Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing.This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. ADAR can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication. (Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 136 kDa double-stranded RNA-binding protein; ADA; ADAR1; Adenosine Deaminase; adenosine deaminase acting on RNA 1-A; Double-stranded RNA-specific adenosine deaminase; DRADA; dsRNA adenosine deaminase; dsRNA adeonosine deaminase; IFI-4; interferon-induced protein 4; Interferon-inducible protein 4; K88DSRBP; p136; RNA adenosine deaminase 1; RNA-specific adenosine deaminase p110 form; RNA-specific adenosine deaminase p150 form; unnamed protein product
Gene Aliases: ADAR; ADAR1; Adar1p110; Adar1p150; AGS6; AV242451; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; mZaADAR; P136
UniProt ID: (Human) P55265, (Mouse) Q99MU3
Entrez Gene ID: (Human) 103, (Mouse) 56417
DNA binding
RNA binding
double-stranded RNA binding
double-stranded RNA adenosine deaminase activity
adenosine deaminase activity
protein binding
tRNA-specific adenosine deaminase activity
hydrolase activity
metal ion binding
left-handed Z-DNA binding
poly(A) RNA binding
RNA metabolism protein
nucleic acid metabolism protein
osteoblast differentiation
hematopoietic progenitor cell differentiation
immune system process
somatic diversification of immune receptors via somatic mutation
adenosine to inosine editing
RNA processing
mRNA processing
protein import into nucleus
protein export from nucleus
apoptotic process
response to virus
base conversion or substitution editing
erythrocyte differentiation
gene silencing by RNA
pre-miRNA processing
production of miRNAs involved in gene silencing by miRNA
response to interferon-alpha
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of viral genome replication
innate immune response
defense response to virus
definitive hemopoiesis
negative regulation of type I interferon-mediated signaling pathway
hematopoietic stem cell homeostasis
small RNA loading onto RISC
hepatocyte apoptotic process
cellular response to virus
negative regulation of RNA interference
negative regulation of hepatocyte apoptotic process
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
miRNA loading onto RISC involved in gene silencing by miRNA
negative regulation of apoptotic process
negative regulation of viral genome replication
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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