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Invitrogen

ADAR Polyclonal Antibody

View all (25) ADAR1 antibodies

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Product Details

PA5-96839

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Amount

46 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2808641

Product Specific Information

Positive Samples: U-87MG, A549, DU145, HeLa, HepG2; Cellular Location: Cytoplasm, Nucleus, Nucleus, nucleolus

Target Information

Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing.This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. ADAR can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication. (Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 136 kDa double-stranded RNA-binding protein; ADA; ADAR1; Adenosine Deaminase; adenosine deaminase acting on RNA 1-A; Double-stranded RNA-specific adenosine deaminase; DRADA; dsRNA adenosine deaminase; dsRNA adeonosine deaminase; IFI-4; interferon-induced protein 4; Interferon-inducible protein 4; K88DSRBP; p136; RNA adenosine deaminase 1; RNA-specific adenosine deaminase p110 form; RNA-specific adenosine deaminase p150 form; unnamed protein product

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Gene Aliases: ADAR; ADAR1; Adar1p110; Adar1p150; AGS6; AV242451; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; mZaADAR; P136

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UniProt ID: (Human) P55265, (Rat) P55266, (Mouse) Q99MU3

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Entrez Gene ID: (Human) 103, (Rat) 81635, (Mouse) 56417

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Function(s)
DNA binding RNA binding double-stranded RNA binding double-stranded RNA adenosine deaminase activity adenosine deaminase activity protein binding tRNA-specific adenosine deaminase activity hydrolase activity metal ion binding poly(A) RNA binding left-handed Z-DNA binding RNA metabolism protein nucleic acid metabolism protein
Process(es)
osteoblast differentiation hematopoietic progenitor cell differentiation immune system process somatic diversification of immune receptors via somatic mutation adenosine to inosine editing RNA processing mRNA processing protein import into nucleus protein export from nucleus apoptotic process response to virus base conversion or substitution editing erythrocyte differentiation gene silencing by RNA pre-miRNA processing production of miRNAs involved in gene silencing by miRNA response to interferon-alpha negative regulation of protein kinase activity by regulation of protein phosphorylation positive regulation of viral genome replication innate immune response defense response to virus definitive hemopoiesis negative regulation of type I interferon-mediated signaling pathway hematopoietic stem cell homeostasis small RNA loading onto RISC hepatocyte apoptotic process cellular response to virus negative regulation of RNA interference negative regulation of hepatocyte apoptotic process in utero embryonic development transcription, DNA-templated regulation of transcription, DNA-templated miRNA loading onto RISC involved in gene silencing by miRNA negative regulation of apoptotic process negative regulation of viral genome replication
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