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Invitrogen
ADARB1 Polyclonal Antibody
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FIGURE: 1 / 5
ADARB1 Antibody (PA5-101139) in IHC (P)
Immunohistochemistry analysis of ADARB1 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ADARB1 polyclonal antibody (Product # PA5-101139) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-101139
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ADARB1(Accession P78563), corresponding to amino acid residues L490-Q540.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ADARB1.
Target Information
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADAR2; RNA editase; adenosine deaminase, RNA-specific, B1 (homolog of rat RED1); Double-stranded RNA-specific editase 1; dsRNA adenosine deaminase; dsRNA adenosine deaminase DRADA2; homologous to rat RED1; RNA editase; RNA editase A-I; RNA editing deaminase 1; RNA editing deaminase of glutamate receptors; RNA-editing deaminase 1; RNA-editing enzyme 1
Gene Aliases: 1700057H01Rik; ADAR2; ADARB1; AW124433; AW558573; BB220382; D10Bwg0447e; DRABA2; DRADA2; NEDHYMS; RED1
UniProt ID: (Human) P78563, (Rat) P51400, (Mouse) Q91ZS8
Entrez Gene ID: (Human) 104, (Rat) 25367, (Mouse) 110532
RNA binding
double-stranded RNA binding
double-stranded RNA adenosine deaminase activity
mRNA binding
adenosine deaminase activity
protein binding
tRNA-specific adenosine deaminase activity
hydrolase activity
deaminase activity
identical protein binding
metal ion binding
poly(A) RNA binding
RNA metabolism protein
nucleic acid metabolism protein
immune system process
adenosine to inosine editing
RNA processing
mRNA processing
neuromuscular synaptic transmission
negative regulation of cell proliferation
base conversion or substitution editing
facial nerve morphogenesis
hypoglossal nerve morphogenesis
spinal cord ventral commissure morphogenesis
negative regulation of cell migration
multicellular organism growth
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of viral genome replication
innate immune response
neuromuscular process controlling posture
defense response to virus
regulation of cell cycle
innervation
muscle tissue morphogenesis
motor behavior
motor neuron apoptotic process
mRNA modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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