Proteintech

ADCY3 Polyclonal Antibody

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Protocols

Questions & Answers

Tech Support

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ADCY3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed MDCK cells using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ADCY3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 19492-1-AP (ADCY3 antibody) at dilution of 1:100 (under 40x lens).

ADCY3 Antibody in Immunohistochemistry (IHC) — IF result from Dr. Corbit, Kevin. anti-ADCY3 (19492-1-AP) mark the cilium of Mouse embryonic fibroblasts.

ADCY3 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19492-1-AP (ADCY3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ADCY3 Antibody in Immunoprecipitation (IP) — IP result of anti-ADCY3 (IP:19492-1-AP, 4ug; Detection:19492-1-AP 1:500) with MDCK cells lysate 3500ug.

Product Details

19492-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.65 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys of Lepus yarkandensis.

Frontiers in physiology

Luo S,Li Y,Li S,Jiang R,Deng F,Liu G,Zhang J

Sat Jul 16 00:00:00 EDT 2022

Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys of Lepus yarkandensis.

Frontiers in physiology

Luo S,Li Y,Li S,Jiang R,Deng F,Liu G,Zhang J

Sat Jul 16 00:00:00 EDT 2022

Targeting the HDAC6-Cilium Axis Ameliorates the Pathological Changes Associated with Retinopathy of Prematurity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Ran J,Zhang Y,Zhang S,Li H,Zhang L,Li Q,Qin J,Li D,Sun L,Xie S,Zhang X,Liu L,Liu M,Zhou J

Fri Jul 01 00:00:00 EDT 2022

Preferential Expression of Ca²⁺-Stimulable Adenylyl Cyclase III in the Supraventricular Area, including Arrhythmogenic Pulmonary Vein of the Rat Heart.

Biomolecules

Okamoto Y,Aung NY,Tanaka M,Takeda Y,Takagi D,Igarashi W,Ishii K,Yamakawa M,Ono K

Fri May 20 00:00:00 EDT 2022

Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.

Neuroscience research

Di Nardo A,Rühmkorf A,Award P,Brennecke A,Fagiolini M,Sahin M

Tue Mar 01 00:00:00 EST 2022

Hypoglycemic flavonoids from Selaginella tamariscina (P.Beauv.) Spring.

Phytochemistry

Long HP,Liu J,Xu PS,Xu KP,Li J,Tan GS

Tue Mar 01 00:00:00 EST 2022

Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function.

Developmental dynamics : an official publication of the American Association of Anatomists

Gailey CD,Wang EJ,Jin L,Ahmadi S,Brautigan DL,Li X,Xu W,Scott MM,Fu Z

Mon Feb 01 00:00:00 EST 2021

A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons.

STAR protocols

Di Nardo A,Vasquez S,Sahin M

Fri Dec 18 00:00:00 EST 2020

Phenotypic Screen with TSC-Deficient Neurons Reveals Heat-Shock Machinery as a Druggable Pathway for mTORC1 and Reduced Cilia.

Cell reports

Di Nardo A,Lenoël I,Winden KD,Rühmkorf A,Modi ME,Barrett L,Ercan-Herbst E,Venugopal P,Behne R,Lopes CAM,Kleiman RJ,Bettencourt-Dias M,Sahin M

Tue Jun 23 00:00:00 EDT 2020

Aromatherapy: Activating olfactory calcium-sensing receptors impairs renal hemodynamics via sympathetic nerve-mediated vasoconstriction.

Acta physiologica (Oxford, England)

Lee SP,Wu WY,Hsiao JK,Zhou JH,Chang HH,Chien CT

Tue Jan 01 00:00:00 EST 2019

Aromatherapy: Activating olfactory calcium-sensing receptors impairs renal hemodynamics via sympathetic nerve-mediated vasoconstriction.

Acta physiologica (Oxford, England)

Lee SP,Wu WY,Hsiao JK,Zhou JH,Chang HH,Chien CT

Tue Jan 01 00:00:00 EST 2019

Shortened primary cilium length and dysregulated Sonic hedgehog signaling in Niemann-Pick C1 disease.

Human molecular genetics

Canterini S,Dragotto J,Dardis A,Zampieri S,De Stefano ME,Mangia F,Erickson RP,Fiorenza MT

Thu Jun 15 00:00:00 EDT 2017

Shortened primary cilium length and dysregulated Sonic hedgehog signaling in Niemann-Pick C1 disease.

Human molecular genetics

Canterini S,Dragotto J,Dardis A,Zampieri S,De Stefano ME,Mangia F,Erickson RP,Fiorenza MT

Thu Jun 15 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: AC-III; AC3; Adenylate cyclase type 3; Adenylate cyclase type III; Adenylate cyclase, olfactive type; Adenylyl cyclase 3; adenylyl cyclase, type III; ATP pyrophosphate-lyase 3; type III adenylyl cyclase

Gene Aliases: AC-III; AC3; ADCY3; KIAA0511; mKIAA0511

UniProt ID: (Human) O60266, (Rat) P21932, (Mouse) Q8VHH7

Entrez Gene ID: (Human) 109, (Rat) 64508, (Mouse) 104111

Function(s)