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Invitrogen
ADFP Polyclonal Antibody
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FIGURE: 1 / 6
ADFP Antibody (PA5-79830) in IHC (P)
Immunohistochemistry analysis of ADFP on paraffin-embedded human mammary cancer tissue. Sample was incubated with ADFP polyclonal antibody (Product# PA5-79830) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Product Details
PA5-79830
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human ADFP recombinant protein (Position: K226-Q418).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: MCF-7 whole cell. IHC: mouse testis tissue, rat testis tissue, human mammary cancer tissue.
Target Information
Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adipophilin; adipose differentiation related protein; Adipose differentiation-related protein; ADRP; ADRP antibody; MGC10598; Perilipin-2
Gene Aliases: AA407157; ADFP; ADPH; ADRP; PLIN2
UniProt ID: (Human) Q99541, (Mouse) P43883
Entrez Gene ID: (Human) 123, (Mouse) 11520, (Rat) 298199
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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