Invitrogen

ADH5 Polyclonal Antibody

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ADH5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ADH5 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ADH5 polyclonal antibody (Product # PA5-102560) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

ADH5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ADH5 in human prostate tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ADH5 polyclonal antibody (Product # PA5-102560) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ADH5 Antibody in Western Blot (WB) — Western blot analysis of ADH5 in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with ADH5 polyclonal antibody (Product # PA5-102560).

Product Details

PA5-102560

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ADH5, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2851962

Target Information

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADH-B2; alcohol dehydrogenase (class III), chi polypeptide; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; class III alcohol dehydrogenase, chi subunit; epididymis secretory sperm binding protein Li 60p; FALDH; FDH; formaldehyde dehydrogenase; Glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase

Gene Aliases: Adh-2; ADH-3; Adh-5; Adh2; Adh3; ADH5; ADHX; FALDH; FDH; GSH-FDH; GSNOR; HEL-S-60p

UniProt ID: (Human) P11766, (Rat) P12711, (Mouse) P28474

Entrez Gene ID: (Human) 128, (Rat) 100145871, (Mouse) 11532

Molecular Function: dehydrogenase oxidoreductase metabolite interconversion enzyme