Invitrogen

ADH5 Polyclonal Antibody

Product Image Gallery

ADH5 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ADH5 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ADH5 polyclonal antibody (Product # PA5-102560) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

ADH5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ADH5 in human prostate tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ADH5 polyclonal antibody (Product # PA5-102560) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ADH5 Antibody in Western Blot (WB) — Western blot analysis of ADH5 in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with ADH5 polyclonal antibody (Product # PA5-102560).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:500-1:2000

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ADH5, corresponding to a region within C-terminal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2851962

Target Information

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADH-B2; alcohol dehydrogenase (class III), chi polypeptide; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; class III alcohol dehydrogenase, chi subunit; epididymis secretory sperm binding protein Li 60p; FALDH; FDH; formaldehyde dehydrogenase; Glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase

Gene Aliases: Adh-2; ADH-3; Adh-5; Adh2; Adh3; ADH5; ADHX; FALDH; FDH; GSH-FDH; GSNOR; HEL-S-60p

UniProt ID: (Human) P11766, (Rat) P12711, (Mouse) P28474

Entrez Gene ID: (Human) 128, (Rat) 100145871, (Mouse) 11532

Molecular Function: dehydrogenase oxidoreductase reductase