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PA1-041A detects adenosine receptor A1 (A1AR) in rat tissues. This antibody does not detect other AR subtypes. Previous lots of this antibody have been used in human and bovine samples.
PA1-041A has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects an ~37 kDa protein in rat brain representing A1AR.
The PA1-041A immunogen is a synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.
Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adenosine A1 receptor variant 1; adenosine A1 receptor variant 2; Adenosine receptor A1; ADO-A1 receptor
Gene Aliases: ADORA1; RDC7
Molecular Function: G-protein coupled receptor