Invitrogen

ADORA1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

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ADORA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Adenosine Receptor A1 was performed using 90% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (Product # PA3-041) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the membrane. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

ADORA1 Antibody in Western Blot (WB) — Published figure using ADORA1 polyclonal antibody (Product # PA3-041) in Western Blot

ADORA1 Antibody — Figure 1 Generation of a Mouse Model with Tetracycline-Regulated Enhanced Expression of Adenosine A 1 Receptor Specifically in Forebrain Neurons (A) The tetracycline-regulated (Tet-Off) expression of the mouse A 1 receptor (A 1 R). Ca 2+ /calmodulin-dependent kinase II (CaMKII) promoter controls the expression of the tetracycline transactivator (tTA) gene product, which induces the simultaneous transcription of the A 1 R and mCherry specifically in forebrain neurons, by binding to a tetracycline responsive element (TRE) containing a bidirectional promoter (P miniCMV ). Thus, the gene expression could be blocked by tetracycline or its stable analog doxycycline (Dox). (B) Spatial distribution of mCherry expression in the mouse brain (green, neuronal nuclei marker NeuN; red, anti-mCherry/RFP staining). (C) Relative mRNA expression of A 1 R in cerebellum (cer), cortex (ctx), hippocampus (hip), and striatum (str) of wild-type (WT), A1 OFF, A1 mice Dox treated from birth; A1 ON, A1 mice Dox treated from birth, followed by 4 weeks Dox withdrawal, normalized to s12, GAPDH, and actin (n = 4). (D) Representative western blot demonstrating the effect of 4 weeks Dox treatment on mCherry and A 1 R protein expression in different brain regions of WT and A1 mice (n = 4). (E) Densitometric quantification of A 1 R protein expression in different brain regions of WT and TG mice normalized to actin (n = 4). One-way ANOVA with Bonferroni post hoc test: *p < 0.05, **p < 0.01, n.s., not significan

Product Details

PA3-041

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides corresponding to the C-terminal portion of human and mouse Adenosine Receptor A1.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2222210

Product Specific Information

PA3-041 detects Adenosine Receptor A1 from human and mouse samples. Reactivity is stronger in mouse, and is only recommended for use in overexpressed or recombinant human samples.

PA3-041 has been successfully used in Western blot applications. By Western blot PA3-041 detects a ~34-37 kDa band representing Adenosine Receptor A1 from mouse brain.

The PA3-041 immunogen is a mix of synthetic peptides corresponding to the C-terminal portion of human and mouse Adenosine Receptor A1 residues. The immunizing sequences are: C(309) Q P A P P I D E D L P E E R P D D(326) for human, and C(309) Q P K P P I E E D I P E E K A D D(326) for mouse.

Target Information

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.