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Invitrogen
ADORA1 Polyclonal Antibody
FIGURE: 1 / 3
ADORA1 Antibody (PA3-041) in ICC/IF
Immunofluorescence analysis of Adenosine Receptor A1 was performed using 90% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (Product # PA3-041) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, A... View More
Product Details
PA3-041
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptides corresponding to the C-terminal portion of human and mouse Adenosine Receptor A1.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2222210
Product Specific Information
PA3-041 detects Adenosine Receptor A1 from human and mouse samples. Reactivity is stronger in mouse, and is only recommended for use in overexpressed or recombinant human samples.
PA3-041 has been successfully used in Western blot applications. By Western blot PA3-041 detects a ~34-37 kDa band representing Adenosine Receptor A1 from mouse brain.
The PA3-041 immunogen is a mix of synthetic peptides corresponding to the C-terminal portion of human and mouse Adenosine Receptor A1 residues. The immunizing sequences are: C(309) Q P A P P I D E D L P E E R P D D(326) for human, and C(309) Q P K P P I E E D I P E E K A D D(326) for mouse.
Target Information
Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adenosine A1 receptor variant 1; adenosine A1 receptor variant 2; Adenosine receptor A1; ADO-A1 receptor; AR
Gene Aliases: A1-AR; A1AR; A1R; AA1R; ADORA1; AI848715; BB176431; RDC7; Ri
UniProt ID: (Human) P30542, (Mouse) Q60612
Entrez Gene ID: (Human) 134, (Mouse) 11539
G-protein coupled adenosine receptor activity
G-protein coupled receptor binding
purine nucleoside binding
phospholipase C activity
protein binding
heat shock protein binding
G-protein beta/gamma-subunit complex binding
heterotrimeric G-protein binding
protein heterodimerization activity
signal transducer activity
G-protein coupled receptor activity
G-protein coupled receptor
activation of MAPKK activity
temperature homeostasis
response to hypoxia
adenosine receptor signaling pathway
regulation of respiratory gaseous exchange by neurological system process
negative regulation of acute inflammatory response
negative regulation of leukocyte migration
positive regulation of peptide secretion
regulation of glomerular filtration
protein targeting to membrane
phagocytosis
inflammatory response
signal transduction
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
cell-cell signaling
nervous system development
negative regulation of cell proliferation
negative regulation of glutamate secretion
lipid catabolic process
negative regulation of synaptic transmission, GABAergic
positive regulation of nucleoside transport
negative regulation of neurotrophin production
positive regulation of protein dephosphorylation
negative regulation of renal sodium excretion
negative regulation of circadian sleep/wake cycle, non-REM sleep
negative regulation of apoptotic process
positive regulation of potassium ion transport
positive regulation of epidermal growth factor-activated receptor activity
negative regulation of blood pressure
positive regulation of blood pressure
negative regulation of vasodilation
negative regulation of hormone secretion
cognition
detection of temperature stimulus involved in sensory perception of pain
negative regulation of lipid catabolic process
regulation of sensory perception of pain
negative regulation of synaptic transmission, glutamatergic
negative regulation of cardiac muscle contraction
excitatory postsynaptic potential
relaxation of vascular smooth muscle
negative regulation of mucus secretion
apoptotic signaling pathway
negative regulation of long term synaptic depression
G-protein coupled receptor signaling pathway
negative regulation of circadian sleep/wake cycle, sleep
negative regulation of heart contraction
regulation of synaptic plasticity
negative regulation of inflammatory response
regulation of cardiac muscle contraction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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