Invitrogen

ADORA2A Polyclonal Antibody

1 Published Figure

9 References

View all (18) ADORA2A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (1)

ADORA2A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

ADORA2A Antibody (PA1-042) in ICC/IF

Immunofluorescent analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Adenosine Receptor A2a polyclonal antibody (Product # PA1-042) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and... View More

ADORA2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA1-042

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

Assay-dependent

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.69 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2257858

Product Specific Information

PA1-042 detects adenosine receptor A2a (A2aAR) in human, rat, mouse and canine tissues. This antibody does not detect other AR subtypes.

PA1-042 has been successfully used in Western blot, ICC/IF, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~45 kDa protein from canine striatum representing A2aAR. Immunohistochemical staining of A2aAR in human hippocampus with PA1-042 yields a pattern consistent with plasma membrane staining.

The PA1-042 immunogen is a synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.

Target Information

ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Adenosine Receptors Expression in Human Retina and Choroid with Age-related Macular Degeneration.

Journal of ophthalmic & vision research

Goebel CP,Song YS,Zaitoun IS,Wang S,Potter HAD,Sorenson CM,Sheibani N

PA1-042 was used in Immunohistochemistry to find that all four receptor subtypes were expressed in several layers of the retina, and within the retinal pigment epithelium and choroid.

Sat Jun 10 00:00:00 UTC 2023

Adenosine receptors differentially mediate enteric glial cell death induced by Clostridioides difficile Toxins A and B.

Frontiers in immunology

Costa DVS,Shin JH,Goldbeck SM,Bolick DT,Mesquita FS,Loureiro AV,Rodrigues-Jesus MJ,Brito GAC,Warren CA

PA1-042 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that adenosine receptors appear to play a regulatory role in EGCs death and proinflammatory response induced by TcdA and TcdB, and thus may be potential targets of intervention to prevent post-CDI intestinal dysmotility.

Fri Feb 03 00:00:00 UTC 2023

Cell-type specific profiling of histone post-translational modifications in the adult mouse striatum.

Nature communications

Carpenter MD,Fischer DK,Zhang S,Bond AM,Czarnecki KS,Woolf MT,Song H,Heller EA

PA1-042 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to understand combinatorial relationships between neuronal-subtype-specific epigenetic modifications and gene expression.

Tue Dec 13 00:00:00 UTC 2022

A venous-specific purinergic signaling cascade initiated by Pannexin 1 regulates TNFα-induced increases in endothelial permeability.

Science signaling

Maier-Begandt D,Comstra HS,Molina SA,Krüger N,Ruddiman CA,Chen YL,Chen X,Biwer LA,Johnstone SR,Lohman AW,Good ME,DeLalio LJ,Hong K,Bacon HM,Yan Z,Sonkusare SK,Koval M,Isakson BE

PA1-042 was used in Immunohistochemistry to provide a targetable pathway with the potential to promote vein barrier function and prevent the deleterious effects of vascular leak in response to inflammation.

Tue Mar 02 00:00:00 UTC 2021

Methamphetamine Blocks Adenosine A_2A Receptor Activation via Sigma 1 and Cannabinoid CB₁ Receptors.

International journal of molecular sciences

Casanovas M,Reyes-Resina I,Lillo A,Lillo J,López-Arnau R,Camarasa J,Escubedo E,Navarro G,Franco R

PA1-042 was used in Proximity Ligation Assay (PLA) to investigate the impact of methamphetamine on adenosine A2A receptor (A2AR) and cannabinoid CB1 receptor (CB1R) complexes.

Tue Mar 09 00:00:00 UTC 2021

On the Role of Adenosine A2A Receptor Gene Transcriptional Regulation in Parkinson's Disease.

Frontiers in neuroscience

Falconi A,Bonito-Oliva A,Di Bartolomeo M,Massimini M,Fattapposta F,Locuratolo N,Dainese E,Pascale E,Fisone G,D'Addario C

PA1-042 was used in Western Blotting to study the transcriptional regulation of the A2AR gene in human peripheral blood mononuclear cells in Parkinson's disease.

Wed Sep 30 00:00:00 UTC 2020

Abnormal calcium handling in atrial fibrillation is linked to up-regulation of adenosine A2A receptors.

European heart journal

Llach A,Molina CE,Prat-Vidal C,Fernandes J,Casadó V,Ciruela F,Lluís C,Franco R,Cinca J,Hove-Madsen L

PA1-042 was used in western blot to investigate the relationship between calcium signaling and adenosine A receptor level in atrial fibrillation

Tue Mar 01 00:00:00 UTC 2011

Adenosine A 2B receptors modulate cAMP levels and induce CREB but not ERK1/2 and p38 phosphorylation in rat skeletal muscle cells.

Biochemical and biophysical research communications

Lynge J,Schulte G,Nordsborg N,Fredholm BB,Hellsten Y

PA1-042 was used in western blot to investigate the role of adenosine A(2B) on specific signal pathway in rat skeletal muscle cells

Fri Jul 18 00:00:00 UTC 2003

Stimulation of A(2A) adenosine receptor phosphorylation by protein kinase C activation: evidence for regulation by multiple protein kinase C isoforms.

Biochemistry

Palmer TM,Stiles GL

PA1-042 was used in western blot to investigate the mechanism of A(2A) adenosine receptor phosphorylation by protein kinase C activation

Tue Nov 09 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: A2a receptor; Adenosine A2a-receptor; Adenosine receptor A2a; adenosine receptor subtype A2a; AR; hA2aR; unnamed protein product

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Gene Aliases: A2AAR; A2aR; AA2AR; ADENO; ADORA2; ADORA2A; Adora2l1; RDC8

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UniProt ID: (Human) P29274, (Dog) P11617, (Mouse) Q60613, (Rat) P30543

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Entrez Gene ID: (Human) 135, (Dog) 403960, (Mouse) 11540, (Rat) 25369

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Function(s)

G-protein coupled adenosine receptor activity protein binding enzyme binding identical protein binding signal transducer activity G-protein coupled receptor activity type 5 metabotropic glutamate receptor binding protein heterodimerization activity alpha-actinin binding G-protein coupled receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ADORA2A Polyclonal Antibody

ADORA2A Antibody (PA1-042) in ICC/IF

Product Details

PA1-042

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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