Invitrogen

ADORA2A Polyclonal Antibody

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ADORA2A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Adenosine Receptor A2a polyclonal antibody (Product # PA1-042) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

ADORA2A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Adenosine Receptor A2a showing positive staining in the cytoplasm and membrane of paraffin-treated Human placenta tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Adenosine Receptor A2a polyclonal antibody (Product # PA1-042) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ADORA2A Antibody in Western Blot (WB) — Western blot analysis of Adenosine Receptor A2a was performed by loading 25 µg of human placenta (lane 1), HepG2 (lane 2), Hela (lane 3) and mouse liver (lane 4) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with an Adenosine Receptor A2a polyclonal antibody (Product # PA1-042) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~45 kDa.

Product Details

PA1-042

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	Assay-dependent	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Human, Mouse, Rat
Published species	Dog, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.69 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2257858

Product Specific Information

PA1-042 detects adenosine receptor A2a (A2aAR) in human, rat, mouse and canine tissues. This antibody does not detect other AR subtypes.

PA1-042 has been successfully used in Western blot, ICC/IF, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~45 kDa protein from canine striatum representing A2aAR. Immunohistochemical staining of A2aAR in human hippocampus with PA1-042 yields a pattern consistent with plasma membrane staining.

The PA1-042 immunogen is a synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.

Target Information

ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A venous-specific purinergic signaling cascade initiated by Pannexin 1 regulates TNFα-induced increases in endothelial permeability.

Science signaling

Maier-Begandt D,Comstra HS,Molina SA,Krüger N,Ruddiman CA,Chen YL,Chen X,Biwer LA,Johnstone SR,Lohman AW,Good ME,DeLalio LJ,Hong K,Bacon HM,Yan Z,Sonkusare SK,Koval M,Isakson BE

PA1-042 was used in Immunohistochemistry to provide a targetable pathway with the potential to promote vein barrier function and prevent the deleterious effects of vascular leak in response to inflammation.

Tue Mar 02 00:00:00 EST 2021

On the Role of Adenosine A2A Receptor Gene Transcriptional Regulation in Parkinson's Disease.

Frontiers in neuroscience

Falconi A,Bonito-Oliva A,Di Bartolomeo M,Massimini M,Fattapposta F,Locuratolo N,Dainese E,Pascale E,Fisone G,D'Addario C

PA1-042 was used in Western Blotting to study the transcriptional regulation of the A2AR gene in human peripheral blood mononuclear cells in Parkinson's disease.