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ADORA2B Antibody (PA5-103182) in ICC/IF
Immunofluorescent analysis of ADORA2B in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ADORA2B polyclonal antibody (Product # PA5-103182) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-103182
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ADORA2B(Accession P29275), corresponding to amino acid residues A127-E156.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
RRID
AB_2852550
Target Information
ADORA2B encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A2b receptor; Adenosine receptor A2b; HGNC:264; netrin-1 receptor
Gene Aliases: ADORA2; ADORA2B
UniProt ID: (Human) P29275, (Rat) P29276
Entrez Gene ID: (Human) 136, (Rat) 29316
Molecular Function:
G-protein coupled receptor
activation of MAPK activity
adenosine receptor signaling pathway
positive regulation of chronic inflammatory response to non-antigenic stimulus
cellular defense response
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
JNK cascade
excretion
positive regulation of vascular endothelial growth factor production
positive regulation of guanylate cyclase activity
cellular response to extracellular stimulus
positive regulation of chemokine production
positive regulation of interleukin-6 production
positive regulation of mast cell degranulation
cellular protein metabolic process
relaxation of vascular smooth muscle
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of norepinephrine secretion
positive regulation of steroid biosynthetic process
positive regulation of cAMP biosynthetic process
positive regulation of cGMP biosynthetic process
negative regulation of collagen biosynthetic process
positive regulation of catecholamine secretion
regulation of angiogenesis
positive regulation of vasodilation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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