Invitrogen

ADORA2B Polyclonal Antibody

View all (11) ADORA2B antibodies

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Questions & Answers

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ADORA2B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ADORA2B in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ADORA2B polyclonal antibody (Product # PA5-103182) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

ADORA2B Antibody in Western Blot (WB) — Western blot analysis of ADORA2B in PC12 cell lysate (left lane: treated with blocking peptide). Samples were incubated with ADORA2B polyclonal antibody (Product # PA5-103182).

ADORA2B Antibody in Western Blot (WB) — Western blot analysis of ADORA2B in Jurkat cell lysate. Samples were incubated with ADORA2B polyclonal antibody (Product # PA5-103182).

Product Details

PA5-103182

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunocytochemistry (ICC/IF)	1:200	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ADORA2B(Accession P29275), corresponding to amino acid residues A127-E156. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2852550

Target Information

ADORA2B encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A2b receptor; Adenosine receptor A2b; HGNC:264; netrin-1 receptor

Gene Aliases: ADORA2; ADORA2B

UniProt ID: (Human) P29275, (Rat) P29276

Entrez Gene ID: (Human) 136, (Rat) 29316

Molecular Function: G-protein coupled receptor

activation of MAPK activity adenosine receptor signaling pathway positive regulation of chronic inflammatory response to non-antigenic stimulus cellular defense response G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway activation of adenylate cyclase activity JNK cascade excretion positive regulation of vascular endothelial growth factor production positive regulation of guanylate cyclase activity cellular response to extracellular stimulus positive regulation of chemokine production positive regulation of interleukin-6 production positive regulation of mast cell degranulation cellular protein metabolic process relaxation of vascular smooth muscle positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of norepinephrine secretion positive regulation of steroid biosynthetic process positive regulation of cAMP biosynthetic process positive regulation of cGMP biosynthetic process negative regulation of collagen biosynthetic process positive regulation of catecholamine secretion regulation of angiogenesis positive regulation of vasodilation

Process(es)

G-protein coupled adenosine receptor activity G-protein coupled receptor activity