Invitrogen

ADSS Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (4) ADSS antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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ADSS Antibody in Immunofluorescence (IF) — Immunofluorescent staining of ADSS in human cell line U-251 MG shows positivity in plasma membrane and cytoplasm. Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).

ADSS Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ADSS in human testis and skeletal muscle tissues using ADSS Polyclonal Antibody (Product # PA5-55070). Corresponding ADSS RNA-seq data are presented for the same tissues.

ADSS Antibody in Western Blot (WB) — Western blot analysis of ADSS in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).

ADSS Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of ADSS demonstrates antibody specificity. Immunohistochemical analysis of ADSS using anti-ADSS Polyclonal Antibody (Product # PA5-55070), shows significant staining of ADSS in testis and shows minimal or weak staining in skeletal muscle tissues. The relative expression levels of ADSS within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:20-1:50

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ADSS

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2637738

Product Specific Information

Immunogen sequence: KLDGEIIPHI PANQEVLNKV EVQYKTLPGW NTDISNARAF KELPVNAQNY VRFIEDELQI PVKWIGVGKS RESM

Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Target Information

This gene encodes the enzyme adenylosuccinate synthetase which catalyzes the first committed step in the conversion of inosine monophosphate to adenosine monophosphate. A pseudogene of this gene is found on chromosome 17.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: adenylosuccinate synthetase (Ade(-)H-complementing); adenylosuccinate synthetase 2, non muscle; Adenylosuccinate synthetase isozyme 2; Adenylosuccinate synthetase, acidic isozyme; Adenylosuccinate synthetase, liver isozyme; adenylosuccinate synthetase, non-muscle isozyme; adSS 2; AMPSase 2; IMP--aspartate ligase 2; L-type adenylosuccinate synthetase

Gene Aliases: ADEH; ADSS; ADSS 2; ADSS2; AI314886; AS

UniProt ID: (Human) P30520, (Mouse) P46664

Entrez Gene ID: (Human) 159, (Mouse) 11566

Molecular Function: ligase