Proteintech

AEG-1/MTDH Polyclonal Antibody

Product Image Gallery

AEG-1/MTDH Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using MTDH antibody 13860-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

AEG-1/MTDH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

AEG-1/MTDH Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AEG-1/MTDH Antibody in Immunoprecipitation (IP) — IP result of anti-AEG-1/MTDH-Specific (IP:13860-1-AP, 3ug; Detection:13860-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AEG-1/MTDH-Specific Fusion Protein Ag4840

Conjugate

Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Target Information

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC

Gene Aliases: 2610103J23Rik; 3D3; 3D3/Lyric; AEG-1; AEG1; AV353288; D8Bwg1112e; LYRIC; LYRIC/3D3; MTDH

UniProt ID: (Human) Q86UE4, (Mouse) Q80WJ7, (Rat) Q9Z1W6

Entrez Gene ID: (Human) 92140, (Mouse) 67154, (Rat) 170910

Function(s)

negative regulation of transcription from RNA polymerase II promoter positive regulation of autophagy lipopolysaccharide-mediated signaling pathway negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling bicellular tight junction assembly

Process(es)

RNA polymerase II transcription factor binding transcription coactivator activity double-stranded RNA binding protein binding poly(A) RNA binding NF-kappaB binding