Proteintech

AEG-1/MTDH Polyclonal Antibody

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AEG-1/MTDH Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using MTDH antibody 13860-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

AEG-1/MTDH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

AEG-1/MTDH Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AEG-1/MTDH Antibody in Immunoprecipitation (IP) — IP result of anti-AEG-1/MTDH-Specific (IP:13860-1-AP, 3ug; Detection:13860-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

Product Details

13860-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 22 publications 22 publications
Immunohistochemistry (IHC)	-	View 17 publications 17 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AEG-1/MTDH-Specific Fusion Protein Ag4840 (233-582 aa encoded by BC045642) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.35 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Immunogen sequence: EQLTTASFP VGSKKNKGDS HLNVQVSNFK SGKGDSTLQV SSGLNENLTV NGGGWNEKSV KLSSQISAGE EKWNSVSPAS AGKRKAEPSA WSQDTGDANT NGKDWGRSWS DRSIFSGIGS TAEPVSQSTT SDYQWDVSRN QPYIDDEWSG LNGLSSADPN SDWNAPAEEW GNWVDEERAS LLKSQEPIPD DQKVSDDDKE KGEGALPTGK SKKKKKKKKK QGEDNSTAQD TEELEKEIRE DLPVNTSKTR PKQEKAFSLK TISTSDPAEV LVKNSQPIKT LPPATSTEPS VILSKSDSDK SSSQVPPILQ ETDKSKSNTK QNSVPPSQTK SETSWESPKQ IKKKKKARRE T (233-582 aa encoded by BC045642)

Target Information

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Depletion of astrocyte elevated gene-1 suppresses tumorigenesis through inhibition of Akt activity in bladder cancer cells.

American journal of translational research

Zhang L,Yang G,Chen H,Huang Y,Xue W,Bo J

Mon Mar 21 00:00:00 EDT 2022

Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration, and invasiveness, and promotes apoptosis in pancreatic cancer cells.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Yang X,Song S

Mon Apr 01 00:00:00 EDT 2019

Metadherin contributes to epithelial-mesenchymal transition and paclitaxel resistance induced by acidic extracellular pH in nasopharyngeal carcinoma.

Oncology letters

Yu C,Liu Y,Qin Z

Thu Mar 01 00:00:00 EST 2018

Delivery of miR-375 and doxorubicin hydrochloride by lipid-coated hollow mesoporous silica nanoparticles to overcome multiple drug resistance in hepatocellular carcinoma.

International journal of nanomedicine

Xue H,Yu Z,Liu Y,Yuan W,Yang T,You J,He X,Lee RJ,Li L,Xu C

Mon Feb 19 00:00:00 EST 2018

Hypoxia promotes migration/invasion and glycolysis in head and neck squamous cell carcinoma via an HIF-1α-MTDH loop.

Oncology reports

Zhu G,Peng F,Gong W,She L,Wei M,Tan H,Chen C,Zhang D,Li G,Huang D,Zhang X,Liu Y

Wed Nov 01 00:00:00 EDT 2017

miR-217 inhibits laryngeal cancer metastasis by repressing AEG-1 and PD-L1 expression.

Oncotarget

Miao S,Mao X,Zhao S,Song K,Xiang C,Lv Y,Jiang H,Wang L,Li B,Yang X,Yuan Z,Xiu C,Meng H,Sun J

Fri Sep 22 00:00:00 EDT 2017

MiR-375 and Doxorubicin Co-delivered by Liposomes for Combination Therapy of Hepatocellular Carcinoma.

Molecular therapy. Nucleic acids

Fan YP,Liao JZ,Lu YQ,Tian DA,Ye F,Zhao PX,Xiang GY,Tang WX,He XX

Fri Jun 16 00:00:00 EDT 2017

Knockdown of astrocyte elevated gene-1 inhibited cell growth and induced apoptosis and suppressed invasion in ovarian cancer cells.

Gene

Wang J,Chen X,Tong M

Thu Jun 15 00:00:00 EDT 2017

miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma.

Oncology letters

Yan JJ,Chang Y,Zhang YN,Lin JS,He XX,Huang HJ

Mon May 01 00:00:00 EDT 2017

RETRACTED: Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells

Molecular therapy : the journal of the American Society of Gene Therapy

Teng H,Wang P,Xue Y,Liu X,Ma J,Cai H,Xi Z,Li Z,Liu Y

Sat Oct 01 00:00:00 EDT 2016

Upregulation of AEG-1 Involves in Schwann Cell Proliferation and Migration After Sciatic Nerve Crush.

Journal of molecular neuroscience : MN

Wang Y,Zhang W,Zhu X,Wang Y,Mao X,Xu X,Wang Y

Sat Oct 01 00:00:00 EDT 2016

Upregulation of AEG-1 Involves in Schwann Cell Proliferation and Migration After Sciatic Nerve Crush.

Journal of molecular neuroscience : MN

Wang Y,Zhang W,Zhu X,Wang Y,Mao X,Xu X,Wang Y

Sat Oct 01 00:00:00 EDT 2016

DYT-40, a novel synthetic 2-styryl-5-nitroimidazole derivative, blocks malignant glioblastoma growth and invasion by inhibiting AEG-1 and NF-κB signaling pathways.

Scientific reports

Zou M,Duan Y,Wang P,Gao R,Chen X,Ou Y,Liang M,Wang Z,Yuan Y,Wang L,Zhu H

Thu Jun 02 00:00:00 EDT 2016

Bruton's tyrosine kinase inhibitor restrains Wnt signaling in chronic lymphocytic leukemia.

Molecular medicine reports

Li PP,Lu K,Geng LY,Zhou XX,Li XY,Wang X

Wed Jun 01 00:00:00 EDT 2016

AEG-1/MTDH-activated autophagy enhances human malignant glioma susceptibility to TGF-β1-triggered epithelial-mesenchymal transition.

Oncotarget

Zou M,Zhu W,Wang L,Shi L,Gao R,Ou Y,Chen X,Wang Z,Jiang A,Liu K,Xiao M,Ni P,Wu D,He W,Sun G,Li P,Zhai S,Wang X,Hu G

Tue Mar 15 00:00:00 EDT 2016

AEG-1/MTDH-activated autophagy enhances human malignant glioma susceptibility to TGF-β1-triggered epithelial-mesenchymal transition.

Oncotarget

Zou M,Zhu W,Wang L,Shi L,Gao R,Ou Y,Chen X,Wang Z,Jiang A,Liu K,Xiao M,Ni P,Wu D,He W,Sun G,Li P,Zhai S,Wang X,Hu G

Tue Mar 15 00:00:00 EDT 2016

Biological chemistry

Tong L,Wang C,Hu X,Pang B,Yang Z,He Z,He M,Wei L,Chu M

Fri Jan 01 00:00:00 EST 2016

Biological chemistry

Tong L,Wang C,Hu X,Pang B,Yang Z,He Z,He M,Wei L,Chu M

Fri Jan 01 00:00:00 EST 2016

MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.

Oncotarget

Yan JJ,Zhang YN,Liao JZ,Ke KP,Chang Y,Li PY,Wang M,Lin JS,He XX

Tue Oct 06 00:00:00 EDT 2015

MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.

Oncotarget

Yan JJ,Zhang YN,Liao JZ,Ke KP,Chang Y,Li PY,Wang M,Lin JS,He XX

Tue Oct 06 00:00:00 EDT 2015

Metadherin regulation of vascular endothelial growth factor expression is dependent upon the PI3K/Akt pathway in squamous cell carcinoma of the head and neck.

Medicine

Zhu GC,Yu CY,She L,Tan HL,Li G,Ren SL,Su ZW,Wei M,Huang DH,Tian YQ,Su RN,Liu Y,Zhang X

Sun Feb 01 00:00:00 EST 2015

Metadherin regulation of vascular endothelial growth factor expression is dependent upon the PI3K/Akt pathway in squamous cell carcinoma of the head and neck.

Medicine

Zhu GC,Yu CY,She L,Tan HL,Li G,Ren SL,Su ZW,Wei M,Huang DH,Tian YQ,Su RN,Liu Y,Zhang X

Sun Feb 01 00:00:00 EST 2015

AEG-1 is associated with tumor progression in nonmuscle-invasive bladder cancer.

Medical oncology (Northwood, London, England)

Yang G,Zhang L,Lin S,Li L,Liu M,Chen H,Cao M,Liu D,Huang YR,Bo J

Sun Jun 01 00:00:00 EDT 2014

Metadherin regulates metastasis of squamous cell carcinoma of the head and neck via AKT signalling pathway-mediated epithelial-mesenchymal transition.

Cancer letters

Yu C,Liu Y,Tan H,Li G,Su Z,Ren S,Zhu G,Tian Y,Qiu Y,Zhang X

Fri Feb 28 00:00:00 EST 2014

Metadherin regulates metastasis of squamous cell carcinoma of the head and neck via AKT signalling pathway-mediated epithelial-mesenchymal transition.

Cancer letters

Yu C,Liu Y,Tan H,Li G,Su Z,Ren S,Zhu G,Tian Y,Qiu Y,Zhang X

Fri Feb 28 00:00:00 EST 2014

Overexpression of astrocyte-elevated gene-1 is associated with cervical carcinoma progression and angiogenesis.

Oncology reports

Long M,Dong K,Gao P,Wang X,Liu L,Yang S,Lin F,Wei J,Zhang H

Sun Sep 01 00:00:00 EDT 2013

Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma.

International journal of cancer

Liu Y,Su Z,Li G,Yu C,Ren S,Huang D,Fan S,Tian Y,Zhang X,Qiu Y

Thu Aug 01 00:00:00 EDT 2013

Metadherin confers chemoresistance of cervical cancer cells by inducing autophagy and activating ERK/NF-κB pathway.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhang J,Zhang Y,Liu S,Zhang Q,Wang Y,Tong L,Chen X,Ji Y,Shang Q,Xu B,Chu M,Wei L

Thu Aug 01 00:00:00 EDT 2013

Metadherin confers chemoresistance of cervical cancer cells by inducing autophagy and activating ERK/NF-κB pathway.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhang J,Zhang Y,Liu S,Zhang Q,Wang Y,Tong L,Chen X,Ji Y,Shang Q,Xu B,Chu M,Wei L

Thu Aug 01 00:00:00 EDT 2013

AEG-1 overexpression is essential for maintenance of malignant state in human AML cells via up-regulation of Akt1 mediated by AURKA activation.

Cellular signalling

Long M,Hao M,Dong K,Shen J,Wang X,Lin F,Liu L,Wei J,Liang Y,Yang J,Gu F,Zhang H

Sat Jun 01 00:00:00 EDT 2013

Metadherin is a novel prognostic marker for bladder cancer progression and overall patient survival.

Asia-Pacific journal of clinical oncology

Zhou J,Li J,Wang Z,Yin C,Zhang W

Sat Sep 01 00:00:00 EDT 2012

MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo.

Oncogene

He XX,Chang Y,Meng FY,Wang MY,Xie QH,Tang F,Li PY,Song YH,Lin JS

Thu Jul 12 00:00:00 EDT 2012

Prognostic significance of metadherin overexpression in hepatitis B virus-related hepatocellular carcinoma.

Oncology reports

Gong Z,Liu W,You N,Wang T,Wang X,Lu P,Zhao G,Yang P,Wang D,Dou K

Fri Jun 01 00:00:00 EDT 2012

Prognostic significance of metadherin overexpression in hepatitis B virus-related hepatocellular carcinoma.

Oncology reports

Gong Z,Liu W,You N,Wang T,Wang X,Lu P,Zhao G,Yang P,Wang D,Dou K

Fri Jun 01 00:00:00 EDT 2012

Astrocyte elevated gene-1 overexpression in human primary gallbladder carcinomas: an unfavorable and independent prognostic factor.

Oncology reports

Sun W,Fan YZ,Xi H,Lu XS,Ye C,Zhang JT

Tue Nov 01 00:00:00 EDT 2011

Astrocyte elevated gene-1 overexpression in human primary gallbladder carcinomas: an unfavorable and independent prognostic factor.

Oncology reports

Sun W,Fan YZ,Xi H,Lu XS,Ye C,Zhang JT

Tue Nov 01 00:00:00 EDT 2011

Significance of AEG-1 expression in correlation with VEGF, microvessel density and clinicopathological characteristics in triple-negative breast cancer.

Journal of surgical oncology

Li C,Li R,Song H,Wang D,Feng T,Yu X,Zhao Y,Liu J,Yu X,Wang Y,Geng J

Tue Feb 01 00:00:00 EST 2011

Significance of AEG-1 expression in correlation with VEGF, microvessel density and clinicopathological characteristics in triple-negative breast cancer.

Journal of surgical oncology

Li C,Li R,Song H,Wang D,Feng T,Yu X,Zhao Y,Liu J,Yu X,Wang Y,Geng J

Tue Feb 01 00:00:00 EST 2011

Prognostic significance of AEG-1 expression in colorectal carcinoma.

International journal of colorectal disease

Song H,Li C,Li R,Geng J

Fri Oct 01 00:00:00 EDT 2010

Prognostic significance of AEG-1 expression in colorectal carcinoma.

International journal of colorectal disease

Song H,Li C,Li R,Geng J

Fri Oct 01 00:00:00 EDT 2010

Expression of astrocyte elevated gene-1: a novel marker of the pathogenesis, progression, and poor prognosis for endometrial cancer.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Song H,Li C,Lu R,Zhang Y,Geng J

Fri Oct 01 00:00:00 EDT 2010

Expression of astrocyte elevated gene-1: a novel marker of the pathogenesis, progression, and poor prognosis for endometrial cancer.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Song H,Li C,Lu R,Zhang Y,Geng J

Fri Oct 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC

Gene Aliases: 2610103J23Rik; 3D3; 3D3/Lyric; AEG-1; AEG1; AV353288; D8Bwg1112e; LYRIC; LYRIC/3D3; MTDH

UniProt ID: (Human) Q86UE4, (Mouse) Q80WJ7, (Rat) Q9Z1W6

Entrez Gene ID: (Human) 92140, (Mouse) 67154, (Rat) 170910

Function(s)

negative regulation of transcription from RNA polymerase II promoter positive regulation of autophagy lipopolysaccharide-mediated signaling pathway negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling bicellular tight junction assembly

Process(es)

RNA polymerase II transcription factor binding transcription coactivator activity double-stranded RNA binding protein binding poly(A) RNA binding NF-kappaB binding