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Invitrogen
AES Polyclonal Antibody
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Product Details
PA5-20170
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 16 amino acid peptide from near the amino-terminus of human AES.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is 293 cell lysate.
PA5-20170 can be used with blocking peptide PEP-0287.
Target Information
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amino enhancer of split; Amino-terminal enhancer of split; Gp130-associated protein GAM; Grg-5; Groucho-related protein 5; hGAM protein; homologous to Swiss-Prot accession number P16371; Protein ESP1; Protein GRG; related to Drosophila groucho; TLE family member 5; TLE family member 5, transcriptional modulator; unnamed protein product
Gene Aliases: AES; AES-1; AES-2; AL024115; ESP1; GRG; Grg-5; GRG5; TLE5
UniProt ID: (Mouse) P63002, (Human) Q08117
Entrez Gene ID: (Mouse) 14797, (Human) 166
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
Wnt signaling pathway
cellular response to extracellular stimulus
negative regulation of protein binding
regulation of growth
negative regulation of transcription, DNA-templated
negative regulation of response to cytokine stimulus
response to interleukin-1
negative regulation of canonical Wnt signaling pathway
positive regulation of anoikis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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