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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Afadin Antibodies

          Bethyl Laboratories

          AF6 Polyclonal Antibody

          View all (11) Afadin antibodies

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          Cite AF6 Polyclonal Antibody

          • Antibody Testing Data (2)
          AF6 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          AF6 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          AF6 Antibody (A302-199A) in WB

          Detection of human and mouse AF6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AF6 antibody (Product # A302-199A) (lot A302-199A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          AF6 Antibody in Western Blot (WB)
          AF6 Antibody in Immunoprecipitation (IP)
          AF6 Polyclonal Antibody

          Product Details

          A302-199A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-10 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 1475 and 1525 of human AF6 (myeloid/lymphoid or mixed-lineage leukemia translocated to, 4).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          tris citrate/phosphate, pH 7-8

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Target Information

          AFDN belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Afadin; Afadin adherens junction formation factor; ALL1-fused gene from chromosome 6 protein; FLJ34371; myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; Protein AF-6; RP3-431P23.3; RP3-470B24.4

          View more View less

          Gene Aliases: 5033403D15Rik; Af-6; AF6; Afadin; AFDN; Gm314; I-afadin; MLL-AF6; MLLT4; S-afadin

          View more View less

          UniProt ID: (Human) P55196, (Mouse) Q9QZQ1

          View more View less

          Entrez Gene ID: (Human) 4301, (Mouse) 17356

          View more View less

          Function(s)
          protein binding protein C-terminus binding Ras GTPase binding cadherin binding involved in cell-cell adhesion LIM domain binding cell adhesion molecule binding non-motor actin binding protein
          Process(es)
          cell adhesion signal transduction cell-cell signaling adherens junction organization positive regulation of GTPase activity establishment of endothelial intestinal barrier cell-cell adhesion
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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