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AFP Polyclonal Antibody
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FIGURE: 1 / 3
AFP Antibody (600-401-X80) in ICC/IF
Immunofluorescence Microscopy of AFP antibody. Tissue: Rat liver tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AFP antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AFP is secreted into the extracellular space. Staining: AFP as red fluorescent signal.
Product Details
600-401-X80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-AFP antibody was prepared from whole rabbit serum produced by repeated immunizations with an internal 14 amino acid synthetic peptide of human AFP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: a fetoglobulin; a fetoprotein; Alpha fetoglobulin; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; alpha-foetoprotein; chloride intracellular channel 6; fetoglobulin; fetoprotein; unnamed protein product; α fetoglobulin; α fetoprotein
Gene Aliases: AFP; AFPD; FETA; HPAFP
UniProt ID: (Human) P02771, (Mouse) P02772
Entrez Gene ID: (Human) 174, (Rat) 24177, (Mouse) 11576
ovulation from ovarian follicle
apoptotic process
immune response
sexual reproduction
response to nutrient levels
progesterone metabolic process
homeostasis of number of cells
liver development
transport
response to organic substance
pancreas development
organ regeneration
SMAD protein signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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