|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid peptide near the amino terminus of human AFP.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||10 ug/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
A suggested positive control is human liver tissue lysate.
PA5-21004 can be used with blocking peptide PEP-1118.
Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found in individuals with no obvious pathology. AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. AFP levels in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.
PA5-21004 was used in immunocytochemistry to examine how the elasticity of the matrix affects the pluripotency of hPSCs
|Higuchi A,Kao SH,Ling QD,Chen YM,Li HF,Alarfaj AA,Munusamy MA,Murugan K,Chang SC,Lee HC,Hsu ST,Kumar SS,Umezawa A||Scientific reports (5:null)||2015|