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Invitrogen
AFP Monoclonal Antibody (P5B8)
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FIGURE: 1 / 5
AFP Antibody (MIA1305) in ICC/IF
Immunofluorescent analysis of alpha-fetoprotein (green) in the endoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09) using Gibco® KnockOut™ Serum Replacement. After four days in suspension culture, EBs were plated on Geltrex™-coated tissue culture-treated polystyrene plates and continuously cultured for 21 days. EB cultures were then fixed and permeabilized according to the 3-Germ Layer Immunocytochemistry Kit (Product # A25538) and blocked with 3% ... View More
Product Details
MIA1305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
P5B8
Immunogen
Purified AFP from Cord Blood
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MIA1305 can be used for immunofluorescence analysis of AFP in the endoderm derived from human embryonic stem cells. By Western blot, MIA1305 detects endogenous AFP protein in the early hepatocyte-like cells derived from human embryonic stem cells.
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a fetoglobulin; a fetoprotein; Alpha fetoglobulin; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; fetoglobulin; fetoprotein; α fetoglobulin; α fetoprotein
Gene Aliases: AFP; AFPD; FETA; HPAFP
UniProt ID: (Human) P02771
Entrez Gene ID: (Human) 174
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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