Immunofluorescent analysis of HepG2 cells transfected with either HA-tagged or wild-type AGL using an AGL polyclonal antibody (Product # PA5-12142) at a 1:25 dilution. Cells were fixed in formalin and processed for IF using a HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 1479-1510 amino acids from the C-terminal region of human AGL|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.