Immunofluorescent analysis of AGMAT showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using an AGMAT polyclonal antibody (Product # PA5-35849) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 160 and 352 of Agmatinase|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AGMAT (Agmatinase) hydrolyzes agmatine to putrescine and urea. It is highly expressed in liver and kidney and is also found in skeletal muscle, fetal liver, brain, testis, skin and the gastrointestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.