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Product Details
10686-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
AGO2 Fusion Protein Ag1032 (77-377 aa encoded by BC007633)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.16 mg/mL
Storage conditions
-20°C
Product Specific Information
This antibody is specific to EIF2C2. EIF2C2 exists two isoforms and molecular weight of isoforms are 97 kDa and 94 kDa.
Immunogen sequence: TLSNLCLKI NVKLGGVNNI LLPQGRPPVF QQPVIFLGAD VTHPPAGDGK KPSIAAVVGS MDAHPNRYCA TVRVQQHRQE IIQDLAAMVR ELLIQFYKST RFKPTRIIFY RDGVSEGQFQ QVLHHELLAI REACIKLEKD YQPGITFIVV QKRHHTRLFC TDKNERVGKS GNIPAGTTVD TKITHPTEFD FYLCSHAGIQ GTSRPSHYHV LWDDNRFSSD ELQILTYQLC HTYVRCTRSV SIPAPAYYAH LVAFRARYHL VDKEHDSAEG SHTSGQSNGR DHQALAKAVQ VHQDTLRTMY FA (77-377 aa encoded by BC007633)
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or “Slicer” activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AGO1; argonaute 2; Argonaute RISC catalytic component 2; Argonaute2; argonaute2 {ECO:0000255|HAMAP-Rule:MF_03031}; CTA-204B4.6; eIF-2C 2; eIF-2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eIF2C; eIF2C 1; eIF2C 2; eIF2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eukaryotic translation initiation factor 2C, 2; GERp95; Golgi ER protein 95 kDa; hAgo1; hAgo2; mAgo2; PAZ Piwi domain protein; Piwi/Argonaute family protein meIF2C2; PPD; Protein argonaute-2; Protein slicer
Gene Aliases: AGO2; EIF2C2; Kiaa4215
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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