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Invitrogen
AGO2 Recombinant Rabbit Monoclonal Antibody (7C2)
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FIGURE: 1 / 2
AGO2 Antibody (MA5-50110) in ICC/IF
Immunocytochemistry analysis of MCF-7 with AGO2 monoclonal antibody (Product # MA5-50110) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 492-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-50110
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7C2
Immunogen
A synthesized peptide derived from Human AGO2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGO1; ARGONAUTE 2; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; AT1G31280; AtAGO2; eIF-2C 1; eIF-2C 2; eIF2C; eIF2C 1; EIF2C1; Eukaryotic translation initiation factor 2C 2; hAgo1; LOC840016; PAZ Piwi domain protein; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer; T19E23.7; T19E23_7
Gene Aliases: AGO2; EIF2C2
Entrez Gene ID: (Human) 27161
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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