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Invitrogen
AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience™
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FIGURE: 1 / 6
AGO2 Antibody (14-6519-82) in ICC/IF
Immunofluorescent analysis of Ago2 (green) in U-2 OS cells (45% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37 C and blocked with 10% Normal Goat Serum in PBS with 0.1% Triton X-100 for 1 hour at room temperature. Cells were stained with the Ago2 Monoclonal Antibody (Product # 14-6519-82) at a concentration of 1.25 µg/mL or Rat IgG2a Isotype Control at 4 C overnight in blocking buffer followed by a Goat anti-Rat IgG (H+L) Superclonal Sec... View More
Product Details
14-6519-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
AGO2 11A9
Immunogen
Peptide of human N-terminus
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This AGO2 11A9 monoclonal antibody recognizes human Argonaut 2 (Ago2), which has been described as a transcriptional regulator by means of RNA interference and endonucleolytic cleavage. The epitope recognized by AGO2 11A9 detects reduced Ago2 by Western Blot and stains Ago2 in the cytoplasm and nucleus in Immunocytochemistry. Ago2 monoclonal antibody (AGO2 11A9) has been validated for the following applications: Western Blot and Immunocytochemistry; AGO2 11A9 does not cross react with murine Ago2.
Applications Reported: This AGO2 11A9 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence.
Applications Tested: This AGO2 11A9 antibody has been tested by western blot using lysates from different human cancer cell lines and by immunocytochemistry/immunofluorescence using fixed human U-2 OS cells. This may be used at less than or equal to 4 µg/mL for western blot and at less than or equal to 1.25 µg/mL for immunocytochemistry/immunofluorescence.
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AGO1; argonaute 2; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; CTA-204B4.6; eIF-2C 1; eIF-2C 2; eIF2C; eIF2C 1; eIF2C 2; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C, 2; hAgo1; hAgo2; PAZ Piwi domain protein; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer
Gene Aliases: AGO2; EIF2C2; Q10
UniProt ID: (Human) Q9UKV8
Entrez Gene ID: (Human) 27161
RNA 7-methylguanosine cap binding
RNA polymerase II core binding
core promoter binding
double-stranded RNA binding
single-stranded RNA binding
mRNA binding
translation initiation factor activity
endoribonuclease activity
protein binding
protein C-terminus binding
siRNA binding
miRNA binding
poly(A) RNA binding
metal ion binding
endoribonuclease activity, cleaving siRNA-paired mRNA
endoribonuclease activity, cleaving miRNA-paired mRNA
translation initiation factor
transcription, DNA-templated
translation
translational initiation
Wnt signaling pathway, calcium modulating pathway
post-embryonic development
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
siRNA loading onto RISC involved in RNA interference
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
negative regulation of translational initiation
phosphatidylinositol-mediated signaling
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
RNA phosphodiester bond hydrolysis, endonucleolytic
mRNA cleavage involved in gene silencing by siRNA
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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