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Invitrogen
AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience™
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FIGURE: 1 / 6
AGO2 Antibody (14-6519-82) in ICC/IF
Immunofluorescent analysis of Ago2 (green) in U-2 OS cells (45% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37 C and blocked with 10% Normal Goat Serum in PBS with 0.1% Triton X-100 for 1 hour at room temperature. Cells were stained with the Ago2 Monoclonal Antibody (Product # 14-6519-82) at a concentration of 1.25 µg/mL or Rat IgG2a Isotype Control at 4 C overnight in blocking buffer followed by a Goat anti-Rat IgG (H+L) Superclonal Sec... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-6519-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
AGO2 11A9
Immunogen
Peptide of human N-terminus
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This AGO2 11A9 monoclonal antibody recognizes human Argonaut 2 (Ago2), which has been described as a transcriptional regulator by means of RNA interference and endonucleolytic cleavage. The epitope recognized by AGO2 11A9 detects reduced Ago2 by Western Blot and stains Ago2 in the cytoplasm and nucleus in Immunocytochemistry. Ago2 monoclonal antibody (AGO2 11A9) has been validated for the following applications: Western Blot and Immunocytochemistry; AGO2 11A9 does not cross react with murine Ago2.
Applications Reported: This AGO2 11A9 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence.
Applications Tested: This AGO2 11A9 antibody has been tested by western blot using lysates from different human cancer cell lines and by immunocytochemistry/immunofluorescence using fixed human U-2 OS cells. This may be used at less than or equal to 4 µg/mL for western blot and at less than or equal to 1.25 µg/mL for immunocytochemistry/immunofluorescence.
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AGO1; ARGONAUTE 2; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; AT1G31280; AtAGO2; eIF-2C 1; eIF-2C 2; eIF2C; eIF2C 1; EIF2C1; Eukaryotic translation initiation factor 2C 2; hAgo1; LOC840016; PAZ Piwi domain protein; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer; T19E23.7; T19E23_7
Gene Aliases: AGO2; EIF2C2
Entrez Gene ID: (Human) 27161
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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