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Agrisera
AGO2/Argonaute 2 Polyclonal Antibody
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FIGURE: 1 / 1
AGO2/Argonaute 2 Antibody (AS13-2682) in WB
300 µg/well of Arabidopsis thaliana protein from wild type and AGO 1-36 knock out, AGO 1 knockdown mutant (1-25) were extracted by TCA-acetone precipitation (check protocol tab) from floral tissue and saturated in 8 M urea were separated on 15% SDS-PAGE (1 mm thick gel) and blotted for 1 hour to 0.2 µm nitrocellulose at 100 V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin (buffered in TBS) for 1 hr at room temp with agitation. Blot was incubated in the primary antibody at a dilution of 1:250 for an hour at RT wit... View More
Product Details
AS13-2682
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunogen affinity purified IgY
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or 'Slicer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGO1; ARGONAUTE 2; Argonaute1; AT1G31280; AtAGO2; eIF-2C 1; eIF2C; eIF2C 1; EIF2C1; hAgo1; LOC840016; Protein argonaute-1; T19E23.7; T19E23_7
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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