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          • Primary Antibodies ›
          • EIF2S1 Antibodies
          The product (cat # {{productObj.productId}}) that you're looking for has been discontinued and replaced with {{productObj.replacementProductId}}. The product (cat # {{productObj.productId}}) that you're looking for has been discontinued. Please contact Technical Support with any questions.

          Invitrogen

          EIF2S1 Polyclonal Antibody

          Advanced Verification
          7 Published Figures
          13 References
          View all (35) EIF2S1 antibodies

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          Cite EIF2S1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          • Published Figures (7)
          • Advanced Verification (1)
          EIF2S1 Antibody in Immunocytochemistry (ICC/IF)
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          EIF2S1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          EIF2S1 Antibody (AHO1182) in ICC/IF

          Immunofluorescence analysis of EIF2-alpha was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with EIF2-alpha Rabbit Polyclonal Antibody (Product # AHO1182) at 1:250 dilution in1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          EIF2S1 Antibody in Immunocytochemistry (ICC/IF)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody in Western Blot (WB)
          EIF2S1 Antibody

          Product Details

          AHO1182

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 13 publications 13 publications

          Immunocytochemistry (ICC/IF)

          1:250
          -
          Product Specifications

          Published species

          Amphibian, Human, Mouse, Non-human primate, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant eIF2-alpha.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536326

          Target Information

          EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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