Immunofluorescent analysis of AHR showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an AHR polyclonal antibody (Product # PA5-29642) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 126 and 353 of Human AHR|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 1 publications below|
PA5-29642 targets AHR in IHC (P), IF and WB applications and shows reactivity with Human samples.
The PA5-29642 immunogen is recombinant fragment corresponding to a region within amino acids 126 and 353 of Human AHR.
This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Xeno-sensing activity of the aryl hydrocarbon receptor in human pluripotent stem cell-derived hepatocyte-like cells.
PA5-29642 was used in ChIP assay to determine how the aryl hydrocarbon receptor found in pluripotent stem cell-derived hepatocyte-like cells in humans display xeno-sensing activity
|Kim HM,Kim JW,Choi Y,Chun HS,Im I,Han YM,Song CW,Yoon S,Park HJ||Scientific reports (6:null)||2016|